Fabrication and evaluation of a plasmonic biosensor based on silica-coated gold nanorods for highly-sensitive detection of anti-Müllerian hormone

• Published in: Colloid and interface science communications Vol. 61; p. 100795
• Main Authors: Mirshekari, Hamed, Dabirmanesh, Bahareh, Daneshjou, Sara, Khajeh, Khosro
• Format: Journal Article
• Language: English
• Published: Elsevier B.V 01.07.2024
• Subjects: Anti-müllerian hormone; Gold nanorods; LSPR; Nanobiosensor; Silica; LSPR; Gold nanorods; Nanobiosensor; Silica; Anti-müllerian hormone
• ISSN: 2215-0382; 2215-0382
• DOI: 10.1016/j.colcom.2024.100795

Anti-müllerian hormone is a crucial biomarker for reproductive potential, but current detection methods are not cost-effective or time-efficient. In this study, we set out to develop a biosensor using gold nanorods (Au-NRs) coated with a functionalized silica network. The biosensor structure was completed by covalently binding the anti-Müllerian hormone antibody to SiO2@Au-NRs. We confirmed the proper coating of the gold nanorods using HR-TEM, EDS/EDAX, zeta potential, and FT-IR analysis. To assess the sensitivity of SiO2@Au-NRs, we analyzed the LSPR peak position redshift in different concentrations of ethylene glycol. The biosensor was then used to recognize the AMH antigen and determine the limit of detection (LoD) and quantification (LoQ) for the biosensor. Our research has shown that the SiO2@Au-NRs have a suitable thickness. The SiO2@Au-NRs demonstrated impressive sensitivity as a nano biosensor. The AMH antigen identification results indicated that the nano biosensor was highly sensitive. The LoD and LoQ values were 0.086 and 0.262 ng ml−1, respectively, very close to the values obtained by the ELISA kit. Furthermore, LSPR biosensors have reduced detection costs and measurement time. Their high sensitivity makes them excellent candidates for use in diagnostic kits. [Display omitted] •We synthesized and characterized gold nanorods that have a thin silica coating.•The coated‑gold nanorods were modified to allow anti-Mullerian hormone antibodies to be attached covalently.•The biosensor can detect with high sensitivity and specificity without the need for labels.•The LoD and LoQ values were 0.086 and 0.262 ng ml−1, respectively, very close to the values obtained by the ELISA kit.•This method allows for an anti-Müllerian hormone assay to be performed quickly and inexpensively.