Enhancement of selectivity for producing gamma-cyclodextrin

• Published in: Applied biochemistry and biotechnology Vol. 84; no. 1-9; pp. 955 - 962
• Main Authors: Matioli, G, Zanin, G.M, Moraes, F.F. de
• Format: Journal Article Conference Proceeding
• Language: English
• Published: Heidelberg Springer 2000
• Subjects: Bacillus - enzymology; Bacillus firmus; Bioconversions. Hemisynthesis; Biological and medical sciences; Biotechnology; cassava; corn; corn starch; Cyclodextrins - biosynthesis; Cyclodextrins - chemical synthesis; cyclomaltodextrin glucanotransferase; Fundamental and applied biological sciences. Psychology; gamma-cyclodextrin; gamma-Cyclodextrins; Glucosyltransferases - metabolism; glycyrrhizin; Kinetics; maltodextrins; Manihot; Methods. Procedures. Technologies; Oryza; Polysaccharides - metabolism; potatoes; rice starch; Solanum tuberosum; starch; Starch - metabolism; Substrate Specificity; Zea mays; Enzyme; Transferases; Glycosyltransferases; Selectivity; Bacillaceae; Corn starch; Cyclodextrin; Bacillales; Hydrolysate; Maltodextrin; Production; Bacteria; Cyclomaltodextrin glucanotransferase; Hexosyltransferases
• ISSN: 0273-2289; 1559-0291; 0273-2289
• DOI: 10.1385/ABAB:84-86:1-9:955

The production of cyclodextrins (CDs) by cyclodextrin-glycosyl-transferase (CGTase) from Bacillus firmus was studies, with respect to the effect of the source of starch upon CD yield and on the selectivity for producing gamma-CD. Cyclodextrin production tests were run for 24 h at 50 degrees C, pH 8.0, and 1 mg/L of CGTase, and substrates were maltodextrin or the starches of rice, potato, cassava, and corn hydrolyzed up to D.E. 10. Cornstarch was the best substrate for producing gamma-CD. Later, glycyrrhizin (2.5% [w/v]), which forms a stable complex with gamma-CD, was added to the cornstarch reaction medium and increased the yield of gamma-CD to about four times that produced with only maltodextrin, but the total yield of CDs remained practically unchanged. Therefore, the results showed that the studied CGTase is capable of giving relatively high yield of gamma-CD in the presence of glycyrrhizin as complexant and cornstarch as substrate.