Differential regulation of hypothalamic neuropeptide Y hnRNA and mRNA during psychological stress and insulin-induced hypoglycemia

Many signals reflecting energy balance and stress are integrated at the hypothalamic orexigenic NPY neurons. To determine transcriptional changes of the NPY gene in response to stress, we followed the time course and compared the expression of heteronuclear (hn)- and messenger (m)RNA levels by in si...

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Published inMolecular and cellular endocrinology Vol. 321; no. 2; pp. 138 - 145
Main Authors Ferenczi, Szilamér, Zelei, Edina, Pintér, Bernadett, Szőke, Zsuzsanna, Kovács, Krisztina J.
Format Journal Article
LanguageEnglish
Published Ireland Elsevier Ireland Ltd 10.06.2010
Subjects
Animals
Blood glucose
Blood Glucose - chemistry
Fasting
Gene Expression Regulation
Hypoglycemia
Hypoglycemia - chemically induced
Hypoglycemia - metabolism
Hypothalamus - chemistry
Immunohistochemistry
In Situ Hybridization
Insulin
Male
Mice
Neuropeptide Y - genetics
Neuropeptide Y - metabolism
Restraint
Reverse Transcriptase Polymerase Chain Reaction
RNA, Messenger - metabolism
Stress, Psychological - metabolism
Time course
Time Factors
Transcriptional Activation
Up-Regulation
Blood glucose
Time course
Restraint
Mice
Fasting
Hypoglycemia
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Summary:Many signals reflecting energy balance and stress are integrated at the hypothalamic orexigenic NPY neurons. To determine transcriptional changes of the NPY gene in response to stress, we followed the time course and compared the expression of heteronuclear (hn)- and messenger (m)RNA levels by in situ hybridization histochemistry and by real time PCR in mice following insulin-induced hypoglycemia and restraint. Hypoglycemia in fasted mice resulted in a rapid increase of NPY hnRNA that peaked at 1 h, declined thereafter by 2–4 h after insulin injection and run parallel to that of NPY mRNA. Throughout the time course examined, NPY expressing cells in the medial basal hypothalamus remained overwhelmingly localized to the arcuate nucleus. Following restraint NPY mRNA slightly increased, however hnRNA levels decreased up to 2 h, suggesting increased stability of mature NPY mRNA. These results highlight rapid changes and differential regulation of NPY expression in response to metabolic and stress challenges.
Bibliography:ObjectType-Article-1
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ISSN:0303-7207
1872-8057
DOI:10.1016/j.mce.2010.02.036