Concanavalin A-bound selenoprotein in human serum analyzed by graphite furnace atomic absorption spectrometry

• Published in: Clinical chemistry (Baltimore, Md.) Vol. 40; no. 1; pp. 62 - 70
• Main Authors: Daher, R, Van Lente, F
• Format: Journal Article
• Language: English
• Published: Washington, DC Am Assoc Clin Chem 01.01.1994; American Association for Clinical Chemistry
• Subjects: Adult; Arthritis, Rheumatoid - blood; Biological and medical sciences; Chromatography, Affinity; Chromatography, Gel; Concanavalin A - metabolism; Diabetes Mellitus - blood; Glutathione Peroxidase - blood; Glycosylation; Humans; Investigative techniques, diagnostic techniques (general aspects); Kidney Diseases - blood; Medical sciences; Middle Aged; Miscellaneous. Technology; Neoplasms - blood; Orosomucoid - analysis; Orosomucoid - metabolism; Pathology. Cytology. Biochemistry. Spectrometry. Miscellaneous investigative techniques; Proteins - analysis; Proteins - metabolism; Reference Values; Selenium - administration & dosage; Selenium - blood; Selenoproteins; Spectrophotometry, Atomic; Human; Biological fluid; Trace element; Selenoprotein; Affinity chromatography; Clinical biology; Risk factor; Gel filtration; Biological marker; Serum; Selenium; Quantitative analysis
• ISSN: 0009-9147; 1530-8561
• DOI: 10.1093/clinchem/40.1.62

We developed an assay for the direct determination of selenium in serum with a Perkin-Elmer Model 4100ZL Zeeman atomic absorption spectrometer and Ag-Cu-Mg modifier. We used this assay to analyze concanavalin A-bound selenoprotein (CABSP) in human serum after concanavalin A (ConA) affinity chromatography. The CABSP was identified as a single-chain glycoprotein of 57.3 kDa. Carbohydrate units were N- and O-linked to the protein. The selenium moiety was selenocysteine. Total selenium, glutathione peroxidase (GPX; EC 1.11.1.9), ConA-bound selenium (CABS), and alpha 1-acid glycoprotein (AAG) were determined in normal subjects and patients with various pathological conditions. CABS accounted for 44.1% +/- 6.3% of total selenium in sera from normal subjects and 46.5% +/- 3.9% to 55.1% +/- 8.1% in sera from patients with a variety of diseases. Total selenium in serum was well correlated with serum CABS (r = 0.860), but not with serum GPX activity (r = 0.117), for all patients studied. Serum CABS increased in normal subjects after selenium supplementation. Serum CABSP did not behave as an acute-phase reactant, compared with AAG.