Optimization of (–)-cubebin biotransformation to (–)-hinokinin by the marine fungus Absidia coerulea 3A9

• Published in: Archives of microbiology Vol. 203; no. 7; pp. 4313 - 4318
• Main Authors: de Souza, Jonathan Messias, Santos, Mario Ferreira Conceição, Pedroso, Rita Cassia Nascimento, Pimenta, Leticia Pereira, Siqueira, Kátia Aparecida, Soares, Marcos Antonio, Dias, Gustavo Muniz, Pietro, Rosemeire Cristina Linhari Rodrigues, Ramos, Henrique Pereira, Silva, Marcio Luis Andrade, Pauletti, Patricia Mendonça, Veneziani, Rodrigo Cassio Sola, Ambrósio, Sérgio Ricardo, Braun, Glaucia Hollaender, Januário, Ana Helena
• Format: Journal Article
• Language: English
• Published: Berlin/Heidelberg Springer Berlin Heidelberg 01.09.2021; Springer Nature B.V
• Subjects: Absidia; Absidia coerulea; Archives & records; Biochemistry; Biomedical and Life Sciences; Biotechnology; Biotransformation; Cell Biology; Chemical analysis; Chromatography; Design of experiments; Design optimization; Ecology; Experimental design; Experiments; Fungi; High performance liquid chromatography; Life Sciences; Liquid chromatography; Microbial Ecology; Microbiology; Natural products; Original Paper; Seawater; Solvents; Substrates; Water analysis; Plackett–Burman; Marine fungi; (–)-hinokinin
• ISSN: 0302-8933; 1432-072X
• DOI: 10.1007/s00203-021-02417-0

The genus Absidia is widely used in the biotransformation of different classes of natural products. This study evaluates the ability of the Absidia coerulea 3A9 marine derived strain isolated from the ascidian Distaplia stilyfera to perform biotransformations by conducting assays with (–)-cubebin, as substrate. The experiment was optimized using the experimental design proposed by Plackett–Burman for seven factors and eight experiments, to establish the biotransformation conditions that would allow maximum production of biotransformed dibenzylbutyrolactone (–)-hinokinin. An analytical method based on Reverse-Phase-High Performance Liquid Chromatography (RP-HPLC) was developed to quantify the fungal biotransformation product. The factor that influenced the (–)-hinokinin peak area the most positively was the percentage of seawater (%seawater) given that its %relative standard deviation (%RSD) showed a 32.92% deviation from the real value.