Sensitive and highly selective colorimetric biosensing of vitamin-C and vitamin-B1 by flavoring agent-based silver nanoparticles

• Published in: Journal of biological inorganic chemistry Vol. 27; no. 4-5; pp. 471 - 483
• Main Authors: Naqvi, Syeda Sumra, Anwar, Humera, Siddiqui, Asma, Shah, Muhammad Raza
• Format: Journal Article
• Language: English
• Published: Cham Springer International Publishing 01.08.2022; Springer Nature B.V
• Subjects: Ascorbic acid; Biochemistry; Biomedical and Life Sciences; Biosensors; Colorimetry; Life Sciences; Microbiology; Nanoparticles; Original Paper; Silver; Thiamine; Vitamin C; Vitamin E; AgNPs; Vitamin B1; Biosensor; Vitamin C; Colorimetric detection
• ISSN: 1432-1327; 0949-8257; 1432-1327
• DOI: 10.1007/s00775-022-01944-5

A sensitive scheme was established for the detection of vitamin C (Ascorbic acid) and vitamin B1 (Thiamin HCl) using Maltol capped AgNPs (McAgNPs) as colorimetric sensor. The designed scheme showed an instant alteration in color from yellow to orange and green for vitamin-C and vitamin B1 sequentially. The probe was sensitive in a concentration range of (0–1 µM) with limit of detection 0.064 and 0.038 µM for vitamin C and vitamin B1 sequentially. The interaction mechanism between vitamin C and vitamin B1 and McAgNPs was evaluated by visible spectroscopy, FTIR, and AFM. Vitamin C attaches on the surface of nanoparticles by C=O group, while OH, C–S–C, and NH 2 groups are involved in the binding of vitamin B1 with McAgNPs. The Vit-C/Vit-B1-McAgNPs complexes were stable over a wide range of pHs. The size of McAgNPs increased after the interaction of vitamin C/vitamin B1 from 30–40 nm to 500 and 400 nm sequentially. The scheme was successfully applied for the detection of vitamin C and vitamin B1 in urine, plasma, water, and commercial pharmaceutical tablets with good recoveries. The scheme was ascertained to be more sensitive than many other formerly described schemes. Graphical abstract