Metabolite analysis by supercritical fluid chromatography

Owing to its favorable properties, such as low viscosity and high diffusivity, a supercritical fluid can be used as the mobile phase in chromatography. Supercritical fluid chromatography (SFC) can provide high-speed and high-resolution separation. Since supercritical carbon dioxide (SCCO(2)), which...

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Bibliographic Details
Published inBioanalysis Vol. 2; no. 1; p. 27
Main Authors Matsubara, Atsuki, Fukusaki, Eiichiro, Bamba, Takeshi
Format Journal Article
LanguageEnglish
Published England 01.01.2010
Subjects
Carbon Dioxide - chemistry
Carotenoids - analysis
Chromatography, Supercritical Fluid - methods
Flavonoids - analysis
Humans
Lipids - analysis
Mass Spectrometry - methods
Metabolomics - instrumentation
Metabolomics - methods
Phenols - analysis
Polyphenols
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Summary:Owing to its favorable properties, such as low viscosity and high diffusivity, a supercritical fluid can be used as the mobile phase in chromatography. Supercritical fluid chromatography (SFC) can provide high-speed and high-resolution separation. Since supercritical carbon dioxide (SCCO(2)), which is generally used as the mobile phase in SFC, is automatically emitted at room temperature, SFC is most commonly used as a preparative method. However, SFC can also be used to perform high-precision biomolecular analysis, especially for hydrophobic metabolites, because of the low polarity of SCCO(2). The use of a mass spectrometer with SFC can widen the scope of application of SFC to bioanalysis. In this review, we summarize practical application of SFC as a tool for the analysis of metabolites in real biological samples.
ISSN:1757-6199
DOI:10.4155/bio.09.160