Integrating Transcriptomics and Metabolomics to Characterize Metabolic Regulation to Elevated CO2 in Chlamydomonas Reinhardtii
With atmospheric CO 2 increasing, a large amount of CO 2 is absorbed by oceans and lakes, which changes the carbonate system and affects the survival of aquatic plants, especially microalgae. The main aim of our study was to explore the responses of Chlamydomonas reinhardtii (Chlorophyceae) to eleva...
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Published in | Marine biotechnology (New York, N.Y.) Vol. 23; no. 2; pp. 255 - 275 |
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Main Authors | , , , , , , , , , , |
Format | Journal Article |
Language | English |
Published |
New York
Springer US
01.04.2021
Springer Nature B.V |
Subjects | |
Online Access | Get full text |
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Summary: | With atmospheric CO
2
increasing, a large amount of CO
2
is absorbed by oceans and lakes, which changes the carbonate system and affects the survival of aquatic plants, especially microalgae. The main aim of our study was to explore the responses of
Chlamydomonas reinhardtii
(Chlorophyceae) to elevated CO
2
by combined transcriptome and metabolome analysis under three different scenarios: control (CK, 400 ppm), short-term elevated CO
2
(ST, 1000 ppm), and long-term elevated CO
2
(LT, 1000 ppm). The transcriptomic data showed moderate changes between ST and CK. However, metabolic analysis indicated that fatty acids (FAs) and partial amino acids (AAs) were increased under ST. There was a global downregulation of genes involved in photosynthesis, glycolysis, lipid metabolism, and nitrogen metabolism but increase in the TCA cycle and β-oxidation under LT. Integrated transcriptome and metabolome analyses demonstrated that the nutritional constituents (FAs, AAs) under LT were poor compared with CK, and most genes and metabolites involved in C and N metabolism were significantly downregulated. However, the growth and photosynthesis of cells under LT increased significantly. Thus,
C. reinhardtii
could form a specific adaptive evolution to elevated CO
2
, affecting future biogeochemical cycles. |
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Bibliography: | ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23 |
ISSN: | 1436-2228 1436-2236 |
DOI: | 10.1007/s10126-021-10021-y |