Determination of glyphosate in foodstuff by one novel chemiluminescence-molecular imprinting sensor

• Published in: Spectrochimica acta. Part A, Molecular and biomolecular spectroscopy Vol. 78; no. 5; pp. 1482 - 1486
• Main Authors: Zhao, Peini, Yan, Mei, Zhang, Congcong, Peng, Ruixue, Ma, Dongsheng, Yu, Jinghua
• Format: Journal Article
• Language: English
• Published: England Elsevier B.V 01.05.2011
• Subjects: Adsorption; Biosensing Techniques - instrumentation; Chemiluminescence; Food Analysis - instrumentation; Glycine - analogs & derivatives; Glycine - analysis; Glycine - chemistry; Glyphosate; Luminescent Measurements - instrumentation; Luminescent Measurements - methods; Microscopy, Electron, Scanning; Microspheres; Molecular Imprinting - instrumentation; Molecularly imprinted; Reference Standards; Regression Analysis; Reproducibility of Results; Sensor; Stereoisomerism; Temperature; Chemiluminescence; Glyphosate; Molecularly imprinted; Sensor
• ISSN: 1386-1425; 1873-3557
• DOI: 10.1016/j.saa.2011.01.037

[Display omitted] ► In the study we establish one novel sensor for determination of glyphosate. ► The sensor combined chemiluminescence technology with molecular imprinting technology. ► Glyphosate-imprinted microspheres were synthesized first and modified on glass sheets. ► The modified glass sheets were used to realize the chemiluminescence reaction. ► The sensor proved to be a useful analytical technology for quick pesticide assay. A novel chemiluminescence (CL) sensor for the determination of glyphosate (GLY) was made up based on molecularly imprinted polymer (MIP). The molecularly imprinted microspheres (MIMs) with a small dimension which possess extremely high surface-to-volume ratio were synthesized using precipitation polymerization with GLY as template. And then the MIMs were modified on glass sheets, which were placed at the bottom of wells of microplate as the recognizer. Subsequently, a highly selective and high throughput chemiluminescence (CL)-molecular imprinting (MI) sensor for detection of GLY was achieved. Influencing factors were investigated and optimized in detail. The method can perform 96 independent measurements sequentially in 10 min and the limit of detection (LOD) for GLY was 0.046 μg mL −1. The relative standard deviation (RSD) for 11 parallel measurements of GLY was 4.68%. The results show that CL-MI sensor can become a useful analytical technology for quick molecular recognition.