Improved accumulation of poly-3-hydroxybutyrate by a recombinant cyanobacterium

Synechococcus sp. PCC7942 was transformed with genes encoding poly-3-hydroxybutyrate(PHB)-synthesizing enzymes from Alcaligenes eutrophus by using a new plasmid vector. Under photoautotrophic and nitrogen-starved conditions, PHB content of the transformant was improved by CO sub(2)-enrichment in spa...

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Bibliographic Details
Published inBiotechnology letters Vol. 20; no. 2; pp. 183 - 186
Main Authors TAKAHASHI, H, MIYAKE, M, TOKIWA, Y, ASADA, Y
Format Journal Article
LanguageEnglish
Published Dordrecht Springer 01.02.1998
Springer Nature B.V
Subjects
Alcaligenes eutrophus
Biological and medical sciences
Biotechnology
Escherichia coli
Fundamental and applied biological sciences. Psychology
Methods. Procedures. Technologies
Microbial engineering. Fermentation and microbial culture technology
Synechococcus
Recombinant microorganism
Culture medium
Genetic transformation
Synechococcus
Butyrate(hydroxy)polymer
Environmental factor
Nitrogen
Optimization
Limiting factor
Cyanobacteria
Microorganism culture
Bacteria
Biological accumulation
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Summary:Synechococcus sp. PCC7942 was transformed with genes encoding poly-3-hydroxybutyrate(PHB)-synthesizing enzymes from Alcaligenes eutrophus by using a new plasmid vector. Under photoautotrophic and nitrogen-starved conditions, PHB content of the transformant was improved by CO sub(2)-enrichment in sparging. Supplement of acetate under nitrogen-starvation enhanced its PHB content by more than 25 %. The mol wt and distribution of PHB from the cyanobacterial transformant were similar to those by Escherichia coli transformant with the same genes as described above.
Bibliography:ObjectType-Article-1
SourceType-Scholarly Journals-1
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ISSN:0141-5492
1573-6776
DOI:10.1023/a:1005392827791