Rhizobium quercicola sp. nov., isolated from the leaf of Quercus variablis in China
Strain DKSPLA3 T , a novel Gram-negative, catalase-positive, oxidase-positive, non-spore-forming, aerobic, non-nitrogen-fixing, non‐motile bacterium was isolated from Quercus variablis leaf, in Zunyi, Guizhou, China. Growth occurred at 4–37 °C (optimum 28 °C), pH 4.0–9.0 (optimum pH 7.0) and up to 4...
Saved in:
Published in | Archives of microbiology Vol. 204; no. 9; p. 596 |
---|---|
Main Authors | , , , , , |
Format | Journal Article |
Language | English |
Published |
Berlin/Heidelberg
Springer Berlin Heidelberg
01.09.2022
Springer Nature B.V |
Subjects | |
Online Access | Get full text |
Cover
Loading…
Summary: | Strain DKSPLA3
T
, a novel Gram-negative, catalase-positive, oxidase-positive, non-spore-forming, aerobic, non-nitrogen-fixing, non‐motile bacterium was isolated from
Quercus variablis
leaf, in Zunyi, Guizhou, China. Growth occurred at 4–37 °C (optimum 28 °C), pH 4.0–9.0 (optimum pH 7.0) and up to 4.0% (w/v) NaCl (optimum under 2.0%, w/v). Phylogeny based on 16S rRNA gene indicated that strain DKSPLA3
T
was a novel species in the genus
Rhizobium
, which was supported by average nucleotide identity (ANI) and digital DNA-DNA hybridization (dDDH) values. The predominant fatty acids of strain DKSPLA3
T
were C
16:0
, C
18:1
ω
7
c
and/or C
18:1
ω
6
c
and C
18:1
ω
7
c
11-methyl. The major respiratory quinone was Q-10. Major polar lipids were diphosphatidyl glycerol (DPG), phosphatidyl glycerol (PG), phosphatidylethanolamine (PE), phosphatidylmonomethylethanolamine (PME), phosphatidylcholine (PC), two unidentified phospholipids (PL) and nine unidentified lipids (L). The genomic G + C content was 64.47 mol%. Based on the phenotypic, phylogenetic and genotypic data, DKSPLA3
T
should be classified as a novel species in the genus
Rhizobium
, for which the name
Rhizobium quercicola
sp. nov. (KCTC 82843
T
= CFCC 16,707T) is proposed. |
---|---|
Bibliography: | ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23 |
ISSN: | 0302-8933 1432-072X |
DOI: | 10.1007/s00203-022-03188-y |