Light-Induced Functional Pinealectomy: Expression of MT2 Receptors in Liver Cells of C57BL/6 Mice after Melatonin Treatment

• Published in: Bulletin of experimental biology and medicine Vol. 173; no. 4; pp. 569 - 574
• Main Authors: Michurina, S. V., Kolesnikov, S. I., Ishchenko, I. Yu, Arkhipov, S. A.
• Format: Journal Article
• Language: English
• Published: New York Springer US 01.08.2022; Springer Nature B.V
• Subjects: Alzheimer's disease; Antibodies; Avidin; Biomedical and Life Sciences; Biomedicine; Biotin; Body weight; Cell Biology; Cell cycle; Enzymes; Estrogens; Experiments; Hepatocytes; Immunohistochemistry; Internal Medicine; Laboratories; Laboratory Medicine; Light; Light effects; Lighting; Liver; Medical research; Melatonin; Melatonin receptors; Metabolism; Pathology; Peroxidase; Physiology; Sleep disorders; melatonin receptor MT; light-induced functional pinealectomy; mice C57BL/6; melatonin; liver
• ISSN: 0007-4888; 1573-8221
• DOI: 10.1007/s10517-022-05590-4

We performed an immunohistochemical study of MT 2 melatonin receptor expression in the liver of C57BL/6 mice with modeled light-induced functional pinealectomy and after melatonin administration by the indirect avidin-biotin peroxidase ABC method. The animals were kept for 14 days under constant lighting. Intragastric administration of melatonin in physiological doses (1 mg/kg body weight for 14 days) to mice with light-induced functional pinealectomy resulted in a 2-fold increase in the relative expression area of MT 2 receptors in liver cells in comparison with that in animals kept under standard lighting conditions, 24-h lighting for 14 days, or 24-h lighting receiving placebo (intragastric administration of 200 ml distilled water). Melatonin treatment had practically no effect on MT 2 staining intensity. Our results attest to the important role of MT 2 receptors in melatonin synthesis disorders and can serve as the basis for the development of therapeutic strategies aimed at melatonin receptors.