Potential probiotic Lacticaseibacillus rhamnosus MTCC-5897 attenuates Escherichia coli induced inflammatory response in intestinal cells

• Published in: Archives of microbiology Vol. 203; no. 9; pp. 5703 - 5713
• Main Authors: Gupta, Taruna, Kaur, Harpreet, Kapila, Suman, Kapila, Rajeev
• Format: Journal Article
• Language: English
• Published: Berlin/Heidelberg Springer Berlin Heidelberg 01.11.2021; Springer Nature B.V
• Subjects: Attenuation; Biochemistry; Biomedical and Life Sciences; Biotechnology; Cell Biology; Competition; Cytokines; E coli; Ecology; Epithelial cells; Epithelium; Escherichia coli; Gastrointestinal diseases; Immunoglobulins; Immunomodulation; Inflammation; Inflammatory response; Interferon; Interleukin 1; Interleukin 8; Intestine; Life Sciences; Microbial Ecology; Microbiology; NF-κB protein; Nuclear transport; Original Paper; Probiotics; Receptors; TLR2 protein; Toll-like receptors; Transcription; Translocation; Tumor necrosis factor-α; Western blotting; Probiotic; NF-κB; Cytokines; Immunomodulation; Intestinal epithelial cells
• ISSN: 0302-8933; 1432-072X
• DOI: 10.1007/s00203-021-02541-x

Probiotics are microbes having tremendous potential to prevent gastrointestinal disorders. In current investigation, immunomodulatory action of probiotic Lacticaseibacillus rhamnosus  MTCC-5897 was studied during exclusion, competition and displacement of  Escherichia coli on intestinal epithelial (Caco-2) cells. The incubation of intestinal cells with Escherichia coli , enhanced downstream signalling and activated nuclear factor kappa B (NF-κB). This significantly increased ( p  < 0.01) the pro-inflammatory cytokines (IL-8, TNF-α, IFN-ϒ) expression. While, incubation of epithelial cells with Lacticaseibacillus rhamnosus during exclusion and competition with Escherichia coli , counteracted these enhanced expressions. The immunomodulatory feature of Lacticaseibacillus rhamnosus was also highlighted with increased ( p  < 0.05) transcription of toll-like receptor-2 (TLR-2) and single Ig IL-1-related receptor (SIGIRR) along with diminished expression of TLR-4. Likewise, attenuation ( p  < 0.05) of E. coli -mediated enhanced nuclear translocation of NF-κB p-65 subunit by Lacticaseibacillus rhamnosus during exclusion was confirmed with western blotting. Thus, present finding establishes the prophylactic potential of Lacticaseibacillus rhamnosus  against exclusion of Escherichia coli in intestinal cells.