Fluorescence lifetime-activated droplet sorting in microfluidic chip systems

We present a highly efficient microfluidic fluorescence lifetime-activated droplet sorting (FLADS) approach as a novel technology for droplet manipulation in lab-on-a-chip devices. In a proof-of-concept study, we successfully applied the approach to sort droplets containing two different fluorescent...

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Bibliographic Details
Published inLab on a chip Vol. 19; no. 3; pp. 403 - 409
Main Authors Hasan, Sadat, Geissler, David, Wink, Konstantin, Hagen, Axel, Heiland, Josef J, Belder, Detlev
Format Journal Article
LanguageEnglish
Published England Royal Society of Chemistry 29.01.2019
Subjects
Chemical compounds
Dielectrophoresis
Droplets
Fluorescence
Lab-on-a-chip
Lifetime
Microfluidics
Substrates
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Summary:We present a highly efficient microfluidic fluorescence lifetime-activated droplet sorting (FLADS) approach as a novel technology for droplet manipulation in lab-on-a-chip devices. In a proof-of-concept study, we successfully applied the approach to sort droplets containing two different fluorescent compounds on the basis of their corresponding fluorescence lifetime. Towards this end, a technical set-up was developed enabling on-the-fly fluorescence lifetime determination of passing droplets. The herein developed LabVIEW program enabled fast triggering of a downstream dielectrophoretic force sorting functionality depending on average fluorescence lifetimes of individual droplets. The approach worked reliably at individual substrate concentrations from 1 nM to 1 mM. This not only allowed reliable sorting of droplets containing species with different fluorescence lifetimes but also enabled differentiation of mixtures in individual droplets.
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ISSN:1473-0197
1473-0189
DOI:10.1039/c8lc01278d