In-plane molecular organization of hydrated single lipid bilayers: DPPC:cholesterol

Understanding the physical properties of cholesterol-phospholipid systems is essential to gain a better knowledge of the function of each membrane constituent. We present a novel, simple and user-friendly setup that allows for the straightforward grazing incidence X-ray diffraction characterization...

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Published inNanoscale Vol. 10; no. 1; pp. 87 - 92
Main Authors Gumí-Audenis, Berta, Costa, Luca, Redondo-Morata, Lorena, Milhiet, Pierre-Emmanuel, Sanz, Fausto, Felici, Roberto, Giannotti, Marina I, Carlà, Francesco
Format Journal Article
LanguageEnglish
Published England Royal Society of Chemistry 07.01.2018
Subjects
1,2-Dipalmitoylphosphatidylcholine - chemistry
Biochemistry, Molecular Biology
Cellular Biology
Cholesterol
Cholesterol - chemistry
Grazing incidence
Life Sciences
Lipid Bilayers - chemistry
Phospholipids
Physical properties
X-Ray Diffraction
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Summary:Understanding the physical properties of cholesterol-phospholipid systems is essential to gain a better knowledge of the function of each membrane constituent. We present a novel, simple and user-friendly setup that allows for the straightforward grazing incidence X-ray diffraction characterization of hydrated individual supported lipid bilayers. This configuration minimizes the scattering from the liquid and allows the detection of the extremely weak diffracted signal of the membrane, enabling the differentiation of the coexisting domains in DPPC:cholesterol single bilayers.
Bibliography:ObjectType-Article-1
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ISSN:2040-3364
2040-3372
DOI:10.1039/c7nr07510c