Optimizing Bioplastic Production of C. necator Under Mixotrophic Fermentation with CO2 and Glucose

• Published in: Waste and biomass valorization Vol. 15; no. 5; pp. 2857 - 2867
• Main Authors: Unaha, Dueanchai, Jaihao, Pongpipat, Unrean, Pornkamol, Champreda, Verawat
• Format: Journal Article
• Language: English
• Published: Dordrecht Springer Netherlands 01.05.2024; Springer Nature B.V
• Subjects: Aeration; Batch culture; Bioplastics; Carbon; Carbon dioxide; Carbon sources; Climate change; Climate change mitigation; Design of experiments; Design optimization; Engineering; Environment; Environmental Engineering/Biotechnology; Experimental design; Fermentation; Glucose; Industrial Pollution Prevention; Original Paper; Production methods; Renewable and Green Energy; Substrates; Waste Management/Waste Technology; valorization; CO; Mixotrophy; Polyhydroxyalkanoate; Design of experiment
• DOI: 10.1007/s12649-023-02330-1
• ISSN: 1877-2641

Purpose Herein, we examined C. necator for its production of bioplastic under mixotrophic fermentation. The mixotrophic process utilized dual carbon sources of mixed CO 2 and glucose for the production of PHB. Methods C. necator was optimized through adaptive laboratory evolution under a mixed carbon sources of CO 2 and glucose. The isolated mutant was then studied for its ability to co-utilize glucose and CO 2 carbon sources for growth and for PHB production. Experimental design based on central composition design was implemented to optimize PHB production under mixotrophic fermentation. Parameters effecting PHB accumulation including CO 2 and glucose substrate ratio, cell dosage and aeration were studied. Results Under optimized mixotrophic batch process, 0.22 g/L of PHB and 28% PHB content was reached from mixed carbon sources. Further CO 2 and glucose co-feeding strategy optimization in mixotrophic fed-batch, PHB titer was elevated to 0.41 g/L. Conclusion Overall, this study offered a promising alternative for CO 2 valorization through the mixotrophic conversion of CO 2 and glucose to PHB by C. necator , which could provide basis in future Bio-CCU technology development for climate change mitigation. Graphical Abstract