Optimizing Bioplastic Production of C. necator Under Mixotrophic Fermentation with CO2 and Glucose

Purpose Herein, we examined C. necator for its production of bioplastic under mixotrophic fermentation. The mixotrophic process utilized dual carbon sources of mixed CO 2 and glucose for the production of PHB. Methods C. necator was optimized through adaptive laboratory evolution under a mixed carbo...

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Bibliographic Details
Published inWaste and biomass valorization Vol. 15; no. 5; pp. 2857 - 2867
Main Authors Unaha, Dueanchai, Jaihao, Pongpipat, Unrean, Pornkamol, Champreda, Verawat
Format Journal Article
LanguageEnglish
Published Dordrecht Springer Netherlands 01.05.2024
Springer Nature B.V
Subjects
Aeration
Batch culture
Bioplastics
Carbon
Carbon dioxide
Carbon sources
Climate change
Climate change mitigation
Design of experiments
Design optimization
Engineering
Environment
Environmental Engineering/Biotechnology
Experimental design
Fermentation
Glucose
Industrial Pollution Prevention
Original Paper
Production methods
Renewable and Green Energy
Substrates
Waste Management/Waste Technology
valorization
CO
Mixotrophy
Polyhydroxyalkanoate
Design of experiment
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Summary:Purpose Herein, we examined C. necator for its production of bioplastic under mixotrophic fermentation. The mixotrophic process utilized dual carbon sources of mixed CO 2 and glucose for the production of PHB. Methods C. necator was optimized through adaptive laboratory evolution under a mixed carbon sources of CO 2 and glucose. The isolated mutant was then studied for its ability to co-utilize glucose and CO 2 carbon sources for growth and for PHB production. Experimental design based on central composition design was implemented to optimize PHB production under mixotrophic fermentation. Parameters effecting PHB accumulation including CO 2 and glucose substrate ratio, cell dosage and aeration were studied. Results Under optimized mixotrophic batch process, 0.22 g/L of PHB and 28% PHB content was reached from mixed carbon sources. Further CO 2 and glucose co-feeding strategy optimization in mixotrophic fed-batch, PHB titer was elevated to 0.41 g/L. Conclusion Overall, this study offered a promising alternative for CO 2 valorization through the mixotrophic conversion of CO 2 and glucose to PHB by C. necator , which could provide basis in future Bio-CCU technology development for climate change mitigation. Graphical Abstract
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ISSN:1877-2641
1877-265X
DOI:10.1007/s12649-023-02330-1