Cryo-EM of Aβ fibrils from mouse models find tg-APPArcSwe fibrils resemble those found in patients with sporadic Alzheimer’s disease

• Published in: Nature neuroscience Vol. 26; no. 12; pp. 2073 - 2080
• Main Authors: Zielinski, Mara, Peralta Reyes, Fernanda S., Gremer, Lothar, Schemmert, Sarah, Frieg, Benedikt, Schäfer, Luisa U., Willuweit, Antje, Donner, Lili, Elvers, Margitta, Nilsson, Lars N. G., Syvänen, Stina, Sehlin, Dag, Ingelsson, Martin, Willbold, Dieter, Schröder, Gunnar F.
• Format: Journal Article
• Language: English
• Published: New York Nature Publishing Group 01.12.2023
• Subjects: Alzheimer's disease; Animal models; Brain; Disease; Drug development; Electron microscopes; Electron microscopy; Emission analysis; Fibrils; Hydrogen bonds; Medicine; Microscopy; Neurodegenerative diseases; Neuroimaging; Neurosciences; Positron emission; Positron emission tomography; Presenilin 1; Transgenic animals; Transgenic mice; β-Amyloid
• ISSN: 1097-6256; 1546-1726
• DOI: 10.1038/s41593-023-01484-4

Abstract The use of transgenic mice displaying amyloid-β (Aβ) brain pathology has been essential for the preclinical assessment of new treatment strategies for Alzheimer’s disease. However, the properties of Aβ in such mice have not been systematically compared to Aβ in the brains of patients with Alzheimer’s disease. Here, we determined the structures of nine ex vivo Aβ fibrils from six different mouse models by cryogenic-electron microscopy. We found novel Aβ fibril structures in the APP/PS1, ARTE10 and tg-SwDI models, whereas the human type II filament fold was found in the ARTE10, tg-APP Swe and APP23 models. The tg-APP ArcSwe mice showed an Aβ fibril whose structure resembles the human type I filament found in patients with sporadic Alzheimer’s disease. A detailed assessment of the Aβ fibril structure is key to the selection of adequate mouse models for the preclinical development of novel plaque-targeting therapeutics and positron emission tomography imaging tracers in Alzheimer’s disease.