Cot-based cloning and sequencing of the short arm of wheat chromosome 1B

• Published in: Plant, soil and environment Vol. 53; no. 10; pp. 437 - 441
• Main Authors: Simkova, H.,Akademie Ved, Olomouc (Czech Republic). Ustav Experimentalni Botaniky, Janda, J.,Akademie Ved, Olomouc (Czech Republic). Ustav Experimentalni Botaniky, Hribova, E.,Akademie Ved, Olomouc (Czech Republic). Ustav Experimentalni Botaniky, Safar, J.,Akademie Ved, Olomouc (Czech Republic). Ustav Experimentalni Botaniky, Dolezel, J.,Akademie Ved, Olomouc (Czech Republic). Ustav Experimentalni Botaniky
• Format: Journal Article
• Language: English
• Published: Czech Academy of Agricultural Sciences 01.10.2007
• Subjects: 1 bs; ADN; bac library; BANCO DE GENES; BANQUE DE DONNEES; BANQUE DE GENES; BASES DE DATOS; BLE; cbcs; CHROMATOGRAPHIE; CHROMATOGRAPHY; cot; cot filtration; CROMATOGRAFIA; DNA; GENE BANKS; GENOMAS; GENOME; genome analysis; GENOMES; http://www.fao.org/aos/agrovoc#c_11116; http://www.fao.org/aos/agrovoc#c_1594; http://www.fao.org/aos/agrovoc#c_2347; http://www.fao.org/aos/agrovoc#c_24833; http://www.fao.org/aos/agrovoc#c_27583; http://www.fao.org/aos/agrovoc#c_3224; http://www.fao.org/aos/agrovoc#c_7951; http://www.fao.org/aos/agrovoc#c_8373; NUCLEOTIDE SEQUENCE; SECUENCIA NUCLEOTIDICA; SEQUENCE NUCLEOTIDIQUE; TRIGO; TRITICUM AESTIVUM; wheat; WHEATS
• ISSN: 1214-1178; 1805-9368
• DOI: 10.17221/2195-pse

Sequencing of cereal genomes is not an easy task due to their large size and high content of repetitive DNA sequences. There are two basic approaches to simplify analysis of such genomes: 1) reduced representation approaches, such as EST sequencing, methyl filtration and Cot-based cloning and sequencing; 2) analysis of genomes in a stepwise manner, e.g. through creation of chromosome-specific genomic resources. Combination of both approaches, i.e. Cot-based cloning and sequencing of DNA obtained from a chromosome-arm-specific BAC library, was tested in this work.