Identification of Growth Stage Specific Transcript Profiles in Fusarium Proliferatum (Gibberella Fujikuroi, Mating Population D) by cDNA-AFLP Analysis

A cDNA-AFLP approach was developed to identify differentially regulated genes in mycotoxin-producing and non-producing growth stages of Fusarium proliferatum(Gibberella fujikuori Mating Population-D). Using 160 PCR-primer combinations, a number of fragments which showed strikingly different intensit...

Full description

Saved in:
Bibliographic Details
Published inEuropean journal of plant pathology Vol. 110; no. 5-6; pp. 619 - 625
Main Authors Jeney, A, Beki, E, Mule, G, Hornok, L
Format Journal Article
LanguageEnglish
Published Dordrecht Springer Nature B.V 01.06.2004
Subjects
Amino acid sequence
Amino acids
Cell cycle
Fusarium proliferatum
Genes
Genetics
Gibberella fujikuroi
Growth stage
Homology
Mating
Metabolism
Mycotoxins
Neurospora crassa
polyols
Spore germination
Transcription
Online AccessGet full text

Cover

More Information
Summary:A cDNA-AFLP approach was developed to identify differentially regulated genes in mycotoxin-producing and non-producing growth stages of Fusarium proliferatum(Gibberella fujikuori Mating Population-D). Using 160 PCR-primer combinations, a number of fragments which showed strikingly different intensities depending on growth stage were cloned, subjected to Northern analysis and sequenced. Two fragments, amplified from genes up-regulated during spore germination gave significant sequence homology to an amino acid transporter gene from Neurospora crassa and a GAL4-like transcriptional activator, respectively. Among the cDNAs derived from late growth stage transcripts, fragments of genes involved in polyol metabolism and cell cycle regulation, respectively were identified. Additional cDNAs (without significant homologies to known sequences) were also isolated and these clearly differentiated between mycotoxin-producing and non-producing growth stages of the fungus.[PUBLICATION ABSTRACT]
Bibliography:ObjectType-Article-2
SourceType-Scholarly Journals-1
ObjectType-Feature-1
content type line 23
ObjectType-Article-1
ObjectType-Feature-2
ISSN:0929-1873
1573-8469
DOI:10.1023/B:EJPP.0000032401.78288.d2