Synthesis, Characterization of NR@SiO2/PNIPAm-co-Ppa Composite Nanogel and Study On Its Application in Photodynamic Therapy

• Published in: Journal of fluorescence Vol. 32; no. 2; pp. 771 - 782
• Main Authors: Wang, Zirui, Song, Qiusheng, Zhu, Lin, Zhao, Chengyan, Ma, Haihong
• Format: Journal Article
• Language: English
• Published: New York Springer US 01.03.2022; Springer Nature B.V
• Subjects: Analytical Chemistry; Biochemistry; Biological and Medical Physics; Biomedical and Life Sciences; Biomedicine; Biophysics; Biotechnology; Copolymerization; Energy transfer; Fluorescence; Fourier transforms; Free radical polymerization; Free radicals; In vitro methods and tests; Infrared radiation; Irradiation; Isopropylacrylamide; Light sources; Nanoparticles; Original Article; Photodynamic therapy; Photoluminescence; Photon correlation spectroscopy; Silicon dioxide; Singlet oxygen; Toxicity testing; PNIPAm; Photodynamic therapy; Fluorescence resonance energy transfer; Composite nanogel
• ISSN: 1053-0509; 1573-4994
• DOI: 10.1007/s10895-021-02872-4

In the present study, a novel composite nanogel based on fluorescence resonance energy transfer (FRET) and its application for photodynamic therapy is reported. First of all, nanoparticles of silica doped with Nile Red (NR) were prepared by Stöber method, then they were decorated by γ-methacryloxypropyltrimethoxysilane (MPS) to prepare MPS decorated NR@SiO 2 nanoparticles, and finally they were copolymerized with N-isopropylacrylamide (NIPAm) and Pyropheophorbide-a (Ppa) by free radical copolymerization, and composite nanogel of NR@SiO 2 /PNIPAm- co -Ppa was fabricated. The microstructure of the as-prepared nanogel was characterized by Fourier transform infrared spectrum (FTIR), photoluminescence (PL), UV–Visible spectrophotometer (UV–Vis), dynamic light scattering (DLS) and transmission electron microscopy (TEM). PL spectrum indicated that, under irradiation of visible light source, energy can be transferred from NR to Ppa. UV–Vis spectrum demonstrated that aggregation of Ppa is prevented efficiently and Ppa exists as “monomer” state in the composite nanogel. Under irradiation of laser, singlet oxygen ( 1 O 2 ) can be produced efficiently by excited nanogel. The in vitro cytotoxicity test showed that HeLa cells can be killed by the composite nanogel.