Genome sequence of the novel freshwater Microcystis cyanophage Mwe-Yong1112-1

• Published in: Archives of virology Vol. 167; no. 11; pp. 2371 - 2376
• Main Authors: Cai, Ruqian, Li, Dengfeng, Lin, Wei, Qin, Weinan, Pan, Lingting, Wang, Fei, Qian, Minhua, Liu, Wencai, Zhou, Qin, Zhou, Chengxu, Tong, Yigang
• Format: Journal Article
• Language: English
• Published: Vienna Springer Vienna 01.11.2022; Springer Nature B.V
• Subjects: Annotated Sequence Record; Antibiotics; Biomedical and Life Sciences; Biomedicine; Cyanobacteria; Drug resistance; Genomes; Genomics; Infectious Diseases; Medical Microbiology; Microscopy; Nucleotide sequence; Phages; Proteins; Proteomics; Virology; Virulence
• ISSN: 0304-8608; 1432-8798
• DOI: 10.1007/s00705-022-05542-3

The freshwater cyanophage Mwe-Yong1112-1 was isolated using Microcystis wesenbergii as a host and found to have an icosahedral head, about 45 nm in diameter, and a flexible tail, approximately 133 nm in length and 4.5 nm in width. The complete genome of the cyanophage is 39,679 bp in length with a G+C content of 66.6%. Mwe-Yong1112-1 shared the highest pairwise average nucleotide identity (ANI) value of 67.7% (below the ≥95% boundary to define a species) and the highest nucleotide sequence similarity of 17.48% (below the >70% boundary to define a genus) with the most closely related phage. In a proteomic tree, Mwe-Yong1112-1 and three unclassified phages formed a monophyletic clade between the families Saparoviridae and Pyrstoviridae , but Mwe-Yong1112-1 occupied a separate branch from the other three phages, suggesting that it represents a new evolutionary lineage. This study enriches the available information about freshwater cyanophages.