Comparison of the Toxic Effects of Pristine and Photocatalytically Used TiO2 Nanoparticles in Mice

• Published in: Biological trace element research Vol. 200; no. 5; pp. 2298 - 2311
• Main Authors: Hadei, Mostafa, Rabbani, Shahram, Nabizadeh, Ramin, Mahvi, Amir Hossein, Mesdaghinia, Alireza, Naddafi, Kazem
• Format: Journal Article
• Language: English
• Published: New York Springer US 01.05.2022; Springer Nature B.V
• Subjects: Alanine; Alanine transaminase; Aspartate aminotransferase; Biochemistry; Biomedical and Life Sciences; Biotechnology; C-reactive protein; Creatine; Creatine kinase; Exposure; Heart; Hemoglobin; Irradiation; Kidneys; Kinases; L-Lactate dehydrogenase; Lactate; Lactate dehydrogenase; Lactic acid; Life Sciences; Lymphocytes; Nanoparticles; Nutrition; Oncology; Pathological effects; Phosphates; Photocatalysis; Photodegradation; Physiochemistry; Pollutants; Titanium dioxide; Toxicity; Toxicology; Transaminases; Ultraviolet radiation; Water pollution; Water treatment; Environmental toxicology; Water treatment; Nanotoxicity; nanoparticles; TiO; Inhalation exposure; Toxicological assessment
• ISSN: 0163-4984; 1559-0720
• DOI: 10.1007/s12011-021-02846-4

TiO 2 nanoparticles used in the photocatalytic degradation of pollutants in water treatment processes undergo physiochemical changes; therefore, their toxicological effects may be potentially different from those of the pristine nanoparticles. This study compared the toxic effects of exposure to pristine and photocatalytically used TiO 2 nanoparticles in mice. To obtain used TiO 2 , the nanoparticles were used for photocatalytic degradation of a model pollutant under UV irradiation several times. Two groups of mice were exposed to pristine (PT group) and photocatalytically used TiO 2 (UT group) at three different concentrations (5–20 mg/m 3 ) using whole-body exposure chambers (2 h/day, 5 days/weeks, 4 weeks). Exposure to both pristine and used TiO 2 increased the levels of aspartate aminotransferase (AST), alanine aminotransferase (ALT), alkaline phosphate (ALP), lactate dehydrogenase (LDH), C-reactive protein (CRP), and creatine kinase (CK-MB) significantly. Both exposed groups showed higher levels of WBC, lymphocytes, platelets, hematocrits, hemoglobin, and mean corpuscular volume (MCV) and lower levels of RBC and mean corpuscular hemoglobin concentration (MCHC) in a concentration-dependent manner. In all analyses, there were small non-significant differences between the PT and UT groups. More pathological changes were observed in the lung, kidney, and brain of the UT group, while the PT group showed more pathological effects in the liver and heart. The histological observations indicated that damage was mostly in the form of vascular endothelial injury. These two types of TiO 2 may activate different pathways to promote adverse effects. Further studies are required to evaluate and distinguish the mechanisms through which pristine and used TiO 2 induce toxicity.