Production and Recovery of Exo-polygalacturonase from Umbu (Spondias tuberosa) Residue

• Published in: Waste and biomass valorization Vol. 13; no. 2; pp. 1101 - 1115
• Main Authors: Santiago, Ângela Maria, de Sousa Conrado Oliveira, Líbia, de Oliveira, Paloma Lima, Almeida, Raphael Lucas Jacinto, Santos, Newton Carlos, Galdino, Pablícia Oliveira
• Format: Journal Article
• Language: English
• Published: Dordrecht Springer Netherlands 01.02.2022; Springer Nature B.V
• Subjects: Aspergillus niger; Dehydration; Engineering; Environment; Environmental Engineering/Biotechnology; Enzymatic activity; Enzymes; Fermentation; Industrial Pollution Prevention; Original Paper; Polygalacturonase; Potassium; Potassium phosphate; Potassium phosphates; Recovery; Renewable and Green Energy; Residues; Salts; Solid state; Solid state fermentation; Spondias tuberosa; Sugar; Waste Management/Waste Technology; Enzymatic activity; Waste valorisation; Solid-state fermentation
• ISSN: 1877-2641; 1877-265X
• DOI: 10.1007/s12649-021-01551-6

The objective of the present work was to produce exo-polygalacturonase by solid-state fermentation of the dehydrated peels of umbu ( Spondias tuberosa ) using Aspergillus niger CCT 0916 and then, using a biphasic aqueous system, recover the enzyme. The peels were dehydrated at 55 °C to obtain the dried residue and then used to produce the exo-polygalacturonase using Aspergillus niger CCT 0916 and solid-state fermentation. During the 72 h fermentation, the pH, reducing sugars, and the exo-polygalacturonase activity were tested. Then the extracted enzyme was recovered with a biphasic aqueous system composed of PEG/potassium phosphate. The umbu dried residue showed a maximum peak of enzymatic activity with values of 17.30 U/g after 44 h of fermentation. The enzyme extraction using the dried umbu residue yielded a maximum PGA (Activity polygalacturonase) of 32.59 U/g. The biphasic aqueous system composed of PEG8000 and potassium phosphate salts yielded the highest recovery of 97.14%. Graphic Abstract