A Novel Method for the Synthesis of Tc 99m -Ofloxacin Kits Using D-Penicillamine as Coligand and Their Application as Infection Imaging Agent

The employment of radiopharmaceuticals is increasing nowadays for infection imaging and early execution of patients having infectious or inflammatory complaints. The main aim of this study was to discover a novel method for the labeling of ofloxacin with Tc 99m , optimization of labelling conditions...

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Published inBioMed research international Vol. 2015; no. 2015; pp. 1 - 8
Main Authors Anwar, Shahzad, Ahmed, Mahmood, Rasheed, Rashid, Shahzad, Shabnam, Abdul Qadir, Muhammad, Kiran Shahzadi, Syeda
Format Journal Article
LanguageEnglish
Published Cairo, Egypt Hindawi Publishing Corporation 01.01.2015
Hindawi Limited
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Summary:The employment of radiopharmaceuticals is increasing nowadays for infection imaging and early execution of patients having infectious or inflammatory complaints. The main aim of this study was to discover a novel method for the labeling of ofloxacin with Tc 99m , optimization of labelling conditions to get higher percent yield, to assess kits radiochemical purity, in vitro stability, partition coefficient, protein binding, and intracellular accumulation in Pseudomonas aeruginosa, Salmonella typhi, and Escherichia coli in infected rabbits. Maximum labeling efficiency was achieved when 1.5 mg ofloxacin was labeled with 10–20 mCi sodium pertechnetate in the presence of 3 mg D-penicillamine, 75 μg SnCl2. In vitro binding and biodistribution in Pseudomonas aeruginosa, Salmonella typhi, and Escherichia coli showed good results. This new complex is efficient for the imaging of infections caused by Gram-positive and Gram-negative bacteria.
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ISSN:2314-6133
2314-6141
DOI:10.1155/2015/502680