Effects of Dietary Supplementation with κ-Selenocarrageenan on the Selenium Accumulation and Intestinal Microbiota of the Sea Cucumbers Apostichopus japonicus

• Published in: Biological trace element research Vol. 199; no. 7; pp. 2753 - 2763
• Main Authors: Wang, Kai, Liu, Lina, He, Yingying, Qu, Changfeng, Miao, Jinlai
• Format: Journal Article
• Language: English
• Published: New York Springer US 01.07.2021; Springer Nature B.V
• Subjects: Accumulation; Antioxidants; Apostichopus japonicus; Biochemistry; Biomedical and Life Sciences; Biotechnology; Body wall; Capacity; Diet; Dietary supplements; Feeding experiments; Glutathione; Glutathione peroxidase; Holothuroidea; Intestinal microflora; Intestine; Intestines; Life Sciences; Malondialdehyde; Marine invertebrates; Microbiota; Nutrition; Oncology; Peroxidase; Probiotics; Selenium; Survival; Vegetables; Antioxidant capacity; κ-Selenocarrageenan diet; Intestinal microbiota
• ISSN: 0163-4984; 1559-0720
• DOI: 10.1007/s12011-020-02393-4

A 30-day feeding trial was conducted to investigate the effect of κ-selenocarrageenan on the growth performance, selenium accumulation, antioxidant capacity, and intestinal microbiota of sea cucumbers Apostichopus japonicus , with different sizes (70 g ± 10 g and 100 g ± 10 g). Sea cucumbers of each size were randomly assigned into two groups; a diet without supplemented κ-selenocarrageenan was referred to as a control diet, or supplemented with κ-selenocarrageenan at selenium (Se) levels of 2.0 μg/g. Selenium accumulation in the body wall and intestine was determined on days 0, 10, 20, and 30. The survival rate (SR) was significantly higher in the κ-selenocarrageenan-treated group (Se group) than in the control group. After 30 days of feeding, κ-selenocarrageenan supplementation increased the activities of glutathione peroxidase (GSH-Px) and total antioxidant capacity (T-AOC), and decreased malondialdehyde (MDA) levels in A. japonicus . Furthermore, the intestinal microbiota diversity of sea cucumbers was increased by dietary supplementation with κ-selenocarrageenan and the relative abundances of some probiotics (such as Sulfitobacter and Rhodobacteraceae ) were also increased. It is suggested that κ-selenocarrageenan could increase the antioxidant capacity and modulate the intestinal microbiota of sea cucumbers A. japonicus . Further researches will be conducted for its optimal administration concentrations in vivo.