Psychroflexus maritimus sp. nov., isolated from coastal sediment

• Published in: Archives of microbiology Vol. 202; no. 8; pp. 2127 - 2133
• Main Authors: Zhong, Yan-Lin, Zhang, Rui, Zhang, Xiao-Yu, Yu, Li-Xue, Zhao, Meng-Fan, Du, Zong-Jun
• Format: Journal Article
• Language: English
• Published: Berlin/Heidelberg Springer Berlin Heidelberg 01.10.2020; Springer Nature B.V
• Subjects: Biochemistry; Biomedical and Life Sciences; Biotechnology; Catalase; Cell Biology; Deoxyribonucleic acid; DNA; Ecology; Fatty acids; Genomes; Gliding; Life Sciences; Lipids; Menaquinones; Microbial Ecology; Microbiology; New species; Nucleotide sequence; Original Paper; Phosphatidylethanolamine; Phylogenetics; Phylogeny; Quinones; rRNA 16S; Sodium chloride; Draft genome sequencing; Polyphasic taxonomy; 16S rRNA gene
• ISSN: 0302-8933; 1432-072X
• DOI: 10.1007/s00203-020-01933-9

A Gram-stain-negative, strictly aerobic, non-flagellated, gliding, rod-shaped bacterium, designated C1 T was isolated from the coastal sediment of Xiaoshi Island, Weihai, China. Phylogenetic analysis of the 16S rRNA gene and the genome sequence of the newly isolated strain revealed that it belonged to the genus Psychroflexus within the family Flavobacteriaceae . The 16S rRNA gene sequence similarities between strain C1 T and the type strains of Psychroflexus members ranged from 91.6 to 94.9%. The AAI, POCP, ANI and dDDH values between strain C1 T and Psychroflexus torquis ATCC 700755 T were 67.0%, 53.1%, 70.5% and 20.2%, respectively. The DNA G+C content was 34.0 mol%. Strain C1 T grows optimally at 28–30 ℃, at pH 7.5–8.0 and with 3.0–5.0% (w/v) NaCl, and its colonies were orange-colored, convex and circular on the MA plate. Positive for hydrolysis of Tween 20 and catalase activities. The dominant respiratory quinone was menaquinone-6, and the major fatty acids were iso-C 17:0 3-OH and iso-C 15:0 . The polar lipids of strain C1 T consisted of phosphatidylethanolamine and three unidentified lipids. On the basis of phylogenetic, phenotypic and chemotaxonomic data, it is considered that strain C1 T represents a novel species within the genus Psychroflexus , for which, the name Psychroflexus maritimus sp. nov., is proposed. The type strain is C1 T (= MCCC 1H00415 T  = KCTC 72796 T ).