Transcriptomic Analysis of Pacific Oyster (Crassostrea gigas) Zygotes Under Hypotonic Triploid Induction
Polyploid breeding is widely used in various marine species. Low salinity treatment is an effective method of inducing triploid of bivalve mollusks. In this study, RNA-seq was performed to determine genes and pathways involved in hyposaline adaption and cell division of Pacific oyster ( Crassostrea...
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Published in | Journal of Ocean University of China Vol. 20; no. 1; pp. 147 - 158 |
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Main Authors | , , , , , |
Format | Journal Article |
Language | English |
Published |
Heidelberg
Science Press
01.02.2021
Springer Nature B.V Key Laboratory of Mariculture of Ministry of Education, Ocean University of China, Qingdao 266003, China |
Subjects | |
Online Access | Get full text |
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Summary: | Polyploid breeding is widely used in various marine species. Low salinity treatment is an effective method of inducing triploid of bivalve mollusks. In this study, RNA-seq was performed to determine genes and pathways involved in hyposaline adaption and cell division of Pacific oyster (
Crassostrea gigas
) zygotes, trying to better understand the possible molecular mechanism of hypo-osmotic induction. A total of 26965 unigenes were generated in the
de novo
assembly of clean Illumina reads with an average length of 934 bp and N50 of 1721 bp. Of 3024 differentially expressed genes (DEGs), 2501 were up-regulated and 523 were down-regulated. GO (Gene Ontology) annotation and KEGG (Kyoto Encyclopedia of Genes and Genomes) pathway analysis of these DEGs revealed that these DEGs participate a variety of biological processes including osmoregulation, cytoskeleton organization, cell survival and death, and substantially modulate cell proliferation and embryonic development. In summery, RNA-seq methodology was applied for the first time to demonstrate hypotonic-induced transcriptomic alteration in oyster zygotes. Our findings not only interpreted the relatively high mortality of induced larvae, but also provided a valuable reference for further investigations on the mechanism of hyposaline induction, thus should aid to the application of low salinity in triploid induction in large scale aquaculture in future. |
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ISSN: | 1672-5182 1993-5021 1672-5174 |
DOI: | 10.1007/s11802-021-4450-8 |