Blood Pressure Changes After Exposures Increasing Angiotensin-Converting Enzyme Activity and After Its Normalization with Dihydroquercetin in Male Wistar Rats

• Published in: Bulletin of experimental biology and medicine Vol. 166; no. 1; pp. 31 - 34
• Main Authors: Korystova, A. F., Kublik, L. N., Levitman, M. Kh, Shaposhnikova, V. V., Kim, Yu. A., Slashcheva, G. A., Lobanov, A. V., Tukhovskaya, E. A., Korystov, Yu. N.
• Format: Journal Article
• Language: English
• Published: New York Springer US 01.11.2018; Springer Nature B.V
• Subjects: 60 APPLIED LIFE SCIENCES; ANGIOTENSIN; Angiotensin-converting enzyme inhibitors; Animals; AORTA; Biomedical and Life Sciences; Biomedicine; BLOOD PRESSURE; Blood Pressure - drug effects; Cell Biology; DEXAMETHASONE; Dexamethasone - pharmacology; Diuretics; Enzymatic activity; ENZYME ACTIVITY; ENZYME INHIBITORS; ENZYMES; Heart Rate - drug effects; Heart Rate - radiation effects; Hip; HYDROLYSIS; Internal Medicine; Ionizing radiation; Laboratory Medicine; Male; NG-Nitroarginine methyl ester; NG-Nitroarginine Methyl Ester - pharmacology; Nitric oxide; Nitric-oxide synthase; Pathology; Peptidyl-dipeptidase A; Peptidyl-Dipeptidase A - metabolism; Quercetin - analogs & derivatives; Quercetin - pharmacology; Radiation, Ionizing; RATS; Rats, Wistar; X RADIATION; blood pressure; aorta; angiotensin-converting enzyme; dihydroquercetin
• ISSN: 0007-4888; 1573-8221
• DOI: 10.1007/s10517-018-4282-8

Changes in BP and HR were assessed after exposures increasing activity of angiotensin-converting enzyme: ionizing radiation, NO synthase inhibitor (L-NAME), and dexamethasone. Effects of dihydroquercetin and angiotensin-converting enzyme inhibitor enalapril on activity of this enzyme, BP, and HR were also evaluated under these exposures. Wistar male rats were subjected to X-ray irradiation in a dose of 2.5 Gy. Angiotensin-converting enzyme activity in the aorta sections was determined by Hip-His-Leu hydrolysis. BP and HR were recorded using a non-invasive tail-cuff method and PowerLab 8/35 software. BP and HR were not altered with the increase in activity of angiotensin-converting enzyme after irradiation. In case of prolonged (7 days) treatment with NO synthase inhibitor and dexamethasone, the increase in enzyme activity was accompanied by elevation of BP and, in the case of NO synthase inhibitor, HR reduction. Dihydroquercetin normalized the enzyme activity and lowered BP, but not to the normal level. Enalapril normalized BP, increased by NO synthase inhibitor solution intake; at the same time, the angiotensinconverting enzyme activity decreased more than 2-fold in comparison with the normal.