3, 4-Dimethylpyrazole phosphate is an effective and specific inhibitor of soil ammonia-oxidizing bacteria

• Published in: Biology and fertility of soils Vol. 57; no. 6; pp. 753 - 766
• Main Authors: Yin, Chang, Fan, Xiaoping, Chen, Hao, Jiang, Yishun, Ye, Mujun, Yan, Guochao, Peng, Hongyun, Wakelin, Steven A., Liang, Yongchao
• Format: Journal Article
• Language: English
• Published: Berlin/Heidelberg Springer Berlin Heidelberg 01.08.2021; Springer Nature B.V
• Subjects: 3,4-dimethylpyrazole phosphate; Abundance; acid soils; Acidic soils; Agriculture; Alkaline soils; Ammonia; Ammonia-oxidizing bacteria; Arable land; Biomedical and Life Sciences; Community composition; community structure; Composition; Emissions; Life Sciences; Nitrification; Nitrosomonas communis; Nitrous oxide; Original Paper; Oxidation; Phosphates; phylotype; Physicochemical processes; Physicochemical properties; Soil; Soil amendment; Soil bacteria; Soil microorganisms; Soil Science & Conservation; Soils; DMPP; Acid soil; 1-Octyne; N; O
• ISSN: 0178-2762; 1432-0789
• DOI: 10.1007/s00374-021-01565-1

A microcosm study was established using three arable soils differing in both physicochemical properties and AOB or AOA dominance of ammonia oxidization. Nitrification and N 2 O emissions were quantified under 10 Pa C 2 H 2 , 1-octyne, or 3,4-dimethylpyrazole phosphate (DMPP), and the community composition and abundance of AOA and AOB were assessed. Amendment of soil with DMPP had a comparable effect to 1-octyne on AOB activities, composition, and N 2 O emissions across soils. By blocking AOB activity, both DMPP and 1-octyne supported higher rates of AOA growth in both the acid and alkaline soils. Approximately, 50% of ammonia-oxidizing activity in the acid soil was attributed to AOB activity by phylotypes affiliated with Nitrosomonas communis . When AOB were blocked by DMPP or 1-octyne across all soils, recovery of AOA communities was associated with the increased abundance of Nitrososphaera viennesis .