Chemical derivatization as a tool for optimizing MS response in sensitive LC-MS/MS bioanalysis and its role in pharmacokinetic studies

Chemical derivatization is the only technique that directly affects the physicochemical property of an analyte in an LC-MS/MS assay platform. On the other side, current MS instruments are extremely sensitive, but still their absolute sensitivity is analyte-dependent. In this review, first, difficult...

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Bibliographic Details
Published inBioanalysis Vol. 4; no. 2; p. 213
Main Author Niwa, Makoto
Format Journal Article
LanguageEnglish
Published England 01.01.2012
Subjects
Chromatography, Liquid - methods
Humans
Pharmaceutical Preparations - analysis
Pharmaceutical Preparations - chemistry
Pharmacokinetics
Tandem Mass Spectrometry - methods
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Summary:Chemical derivatization is the only technique that directly affects the physicochemical property of an analyte in an LC-MS/MS assay platform. On the other side, current MS instruments are extremely sensitive, but still their absolute sensitivity is analyte-dependent. In this review, first, difficulty in analyzing neutral compounds and introducing acidity/basicity and/or proton affinity will be described. Second, the sweet spot of the conventional MS ion source across clogP values is presented. Third, optimization of hydrophilicity/hydrophobicity by derivatization is described. Lastly, development of a new derivatizing reagent specifically designed for LC-MS/MS is described and its significance in pharmacokinetic analysis is discussed.
ISSN:1757-6199
DOI:10.4155/bio.11.306