A homogeneous high-throughput array for the detection and discrimination of influenza A viruses

• Published in: Chem Vol. 8; no. 6; pp. 1750 - 1761
• Main Authors: Dou, Wei-Tao, Wang, Xiang, Liu, Tingting, Zhao, Suwen, Liu, Jiao-Jiao, Yan, Yao, Li, Jun, Zhang, Chi-Yu, Sedgwick, Adam C., Tian, He, Sessler, Jonathan L., Zhou, Dong-Ming, He, Xiao-Peng
• Format: Journal Article
• Language: English
• Published: Elsevier Inc 09.06.2022
• Subjects: carbohydrates; emissive probes; fluorescence; glycan; influenza virus; mutation; pandemic; screening arrays; vaccine; VIEgens; carbohydrates; glycan; mutation; vaccine; VIEgens; emissive probes; fluorescence; influenza virus; SDG3: Good health and well-being; pandemic; screening arrays
• ISSN: 2451-9294; 2451-9294
• DOI: 10.1016/j.chempr.2022.03.012

The transmission of influenza A viruses (IAVs) from animal to human has resulted in a number of pandemics. Here, we report the synthesis of several sialyl-glycan-functionalized vibration-induced emissive probes (VIEgens V1–V10) that differ in their interaction with the relevant IAV hemagglutinin (HA). Collectively, V1–V10 provide a characteristic “fingerprint” for different IAV subtypes. This differential sensing allowed the construction of a glycoVIEgen array that permits detection of IAVs and discrimination against other viral species; it also enables the determination of IAVs specific to individual species, including bird and human, as well as for more than one species. This array allowed preliminary assessments of adenovirus vaccines specific to a given IAV strain to be made. These results highlight the use of a VIEgen scaffold for differential sensing and as a diagnostic system that could find use in low-income “hotspot” regions where early assessments of viral transmission between people, wildlife, and livestock are challenging. [Display omitted] •Introduces sialyl-glycan-functionalized probes with vibration-induced emission features•Details a “glycoVIEgen” array for ratiometric fluorescence sensing of IAVs•Enables discrimination of IAV mutants and initial assessments of new adenovirus vaccines Rapid and easy-to-use diagnostic technologies that enable influenza A virus (IAV) detection and the identification of the host specificity (i.e., animal to human) have an important role to play in risk mitigation. We report a simple and cost-effective approach, termed “glycoVIEgen array,” that allows the simultaneous detection and determination of the host specificity of IAVs. This array successfully enabled the classification of IAV mutants isolated from 22 infected human individuals and the performance of quality-control tests of experimental adenovirus-based IAV vaccines. The present approach could find use in low-income “hotspot” regions where early assessments of viral transmission between people, wildlife, and livestock would permit rapid intervention. We report a high-throughput “glycoVIEgen” array for the simultaneous detection and determination of the host specificity of influenza A viruses (IAVs). Our array system enables the detection of IAVs with discrimination against other viral species and the determination of IAVs specific to individual species. Moreover, our glycoVIEgen array can classify the level of IAV mutations isolated from infected human individuals. Finally, this approach is amenable to performing quality control on developed IAV vaccines and confirming matched hemagglutinin specificities between vaccines and IAV strains.