Accurate determination of DNA in agarose gels using the novel algorithm GelScann(1.0)
GelScann(1.0) is a user-friendly program that accurately quantitates DNA from CCD imaged agarose gels. The algorithm automatically locates lanes, locates bands within a given lane, and quantitates the intensity of each band. GelScann(1.0) uses a statistical method to discriminate DNA from local back...
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Published in | Bioinformatics Vol. 11; no. 2; pp. 187 - 194 |
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Main Authors | , , |
Format | Journal Article |
Language | English |
Published |
Washington, DC
Oxford University Press
01.04.1995
Oxford |
Subjects | |
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Abstract | GelScann(1.0) is a user-friendly program that accurately quantitates DNA from CCD imaged agarose gels. The algorithm automatically locates lanes, locates bands within a given lane, and quantitates the intensity of each band. GelScann(1.0) uses a statistical method to discriminate DNA from local backgrornd fluorescence. The sensitivity of the LaneFinder and BandFinder can be adjusted by the user interacting with GelScann(1.0) 's option box. We demonstrate that GelScann(1.0) can accurately determine DNA concentrations for routine molecular biology applications and determine the relative intensities of PCR amplified fragments for genetic testing. |
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AbstractList | GelScann(1.0) is a user-friendly program that accurately quantitates DNA from CCD imaged agarose gels. The algorithm automatically locates lanes, locates bands within a given lane, and quantitates the intensity of each band. GelScann (1.0) uses a statistical method to discriminate DNA from local background fluorescence. The sensitivity of the LaneFinder and BandFinder can be adjusted by the user interacting with GelScann (1.0)'s option box. We demonstrate that GelScann(1.0) can accurately determine DNA that GelScann(1.0) can accurately determine DNA concentrations for routine molecular biology applications and determine the relative intensities of PCR amplified fragments for genetic testing. GelScann(1.0) is a user-friendly program that accurately quantitates DNA from CCD imaged agarose gels. The algorithm automatically locates lanes, locates bands within a given lane, and quantitates the intensity of each band. GelScann(1.0) uses a statistical method to discriminate DNA from local background fluorescence. The sensitivity of the LaneFinder and BandFinder can be adjusted by the user interacting with GelScann(1.0)'s option box. We demonstrate that GelScann(1.0) can accurately determine DNA concentrations for routine molecular biology applications and determine the relative intensities of PCR amplified fragments for genetic testing. GelScann(1.0) is a user-friendly program that accurately quantitates DNA from CCD imaged agarose gels. The algorithm automatically locates lanes, locates bands within a given lane, and quantitates the intensity of each band. GelScann(1.0) uses a statistical method to discriminate DNA from local backgrornd fluorescence. The sensitivity of the LaneFinder and BandFinder can be adjusted by the user interacting with GelScann(1.0) 's option box. We demonstrate that GelScann(1.0) can accurately determine DNA concentrations for routine molecular biology applications and determine the relative intensities of PCR amplified fragments for genetic testing. |
Author | Gibbs, Richard A. Allain, Kyle M. Metzker, Michael L. |
Author_xml | – sequence: 1 givenname: Michael L. surname: Metzker fullname: Metzker, Michael L. organization: Department of Molecular and Human Genetics, Baylor College of Medicine, Houston, TX 77030, USA – sequence: 2 givenname: Kyle M. surname: Allain fullname: Allain, Kyle M. organization: Department of Molecular and Human Genetics, Baylor College of Medicine, Houston, TX 77030, USA – sequence: 3 givenname: Richard A. surname: Gibbs fullname: Gibbs, Richard A. organization: Department of Molecular and Human Genetics, Baylor College of Medicine, Houston, TX 77030, USA |
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SubjectTerms | AIDS/HIV Algorithms Bacteriophage M13 - genetics Biological and medical sciences DNA, Viral - analysis Electrophoresis, Agar Gel - methods Female Fundamental and applied biological sciences. Psychology General aspects Genetic Carrier Screening HIV Infections - virology HIV-1 - genetics Humans Image Processing, Computer-Assisted Mathematics in biology. Statistical analysis. Models. Metrology. Data processing in biology (general aspects) Microcomputers Muscular Dystrophies - genetics Polymerase Chain Reaction |
Title | Accurate determination of DNA in agarose gels using the novel algorithm GelScann(1.0) |
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