Accurate determination of DNA in agarose gels using the novel algorithm GelScann(1.0)
GelScann(1.0) is a user-friendly program that accurately quantitates DNA from CCD imaged agarose gels. The algorithm automatically locates lanes, locates bands within a given lane, and quantitates the intensity of each band. GelScann(1.0) uses a statistical method to discriminate DNA from local back...
Saved in:
Published in | Bioinformatics Vol. 11; no. 2; pp. 187 - 194 |
---|---|
Main Authors | , , |
Format | Journal Article |
Language | English |
Published |
Washington, DC
Oxford University Press
01.04.1995
Oxford |
Subjects | |
Online Access | Get full text |
Cover
Loading…
Summary: | GelScann(1.0) is a user-friendly program that accurately quantitates DNA from CCD imaged agarose gels. The algorithm automatically locates lanes, locates bands within a given lane, and quantitates the intensity of each band. GelScann(1.0) uses a statistical method to discriminate DNA from local backgrornd fluorescence. The sensitivity of the LaneFinder and BandFinder can be adjusted by the user interacting with GelScann(1.0) 's option box. We demonstrate that GelScann(1.0) can accurately determine DNA concentrations for routine molecular biology applications and determine the relative intensities of PCR amplified fragments for genetic testing. |
---|---|
Bibliography: | ark:/67375/HXZ-9RG4HPHN-K ArticleID:11.2.187 1Present address: Intermedics Inc., 4000 Technology Drive, Angleton, TX 77515, USA istex:75173D64403A8F6001484EB2FED4751325F23E6D ObjectType-Article-2 SourceType-Scholarly Journals-1 ObjectType-Feature-1 content type line 23 ObjectType-Article-1 ObjectType-Feature-2 |
ISSN: | 1367-4803 0266-7061 1460-2059 |
DOI: | 10.1093/bioinformatics/11.2.187 |