Portable Surface Plasmon Resonance Biosensor for Detection of Nucleic Acids
We present a portable surface plasmon resonance (SPR) sensor based on spectroscopy of surface plasmons on a special diffractive structure. The sensor combines a microfluidic cartridge incorporating the special diffractive structure functionalized with DNA probes and a compact SPR reader. We apply th...
Saved in:
Published in | Procedia engineering Vol. 25; pp. 148 - 151 |
---|---|
Main Authors | , , , , , , , |
Format | Journal Article |
Language | English |
Published |
Elsevier Ltd
2011
|
Subjects | |
Online Access | Get full text |
Cover
Loading…
Summary: | We present a portable surface plasmon resonance (SPR) sensor based on spectroscopy of surface plasmons on a special diffractive structure. The sensor combines a microfluidic cartridge incorporating the special diffractive structure functionalized with DNA probes and a compact SPR reader. We apply the sensor to detection of nucleic acids employing two most common immobilization methods: (a) biotinylated probes immobilized using the biotin-streptavidin chemistry and (b) thiolated probes attached directly to the surface of the SPR sensor. It is demonstrated that both these immobilization methods allow detecting short nucleic acids at levels below 100 pM. |
---|---|
ISSN: | 1877-7058 1877-7058 |
DOI: | 10.1016/j.proeng.2011.12.037 |