Microfluidic system for endocrine disrupting chemicals detection in waterish solution

We present a microfluidic cell plate for endocrine disrupting chemicals (EDCs) detection, like estrogenic activity, in waterish solution. This platform technology consists of four independent micro flow units made of polydimethylsiloxane (PDMS) and glass, which enables a selective detection of up to...

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Bibliographic Details
Published inProcedia engineering Vol. 25; pp. 1185 - 1188
Main Authors Cherkouk, C., Rebohle, L., Howitz, S., Skorupa, W.
Format Journal Article
LanguageEnglish
Published Elsevier Ltd 2011
Subjects
biosensor
EDC
estrogen
fluorescence
microfluidic
Si-based light emission
Si-based light emission
estrogen
biosensor
microfluidic
fluorescence
EDC
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Summary:We present a microfluidic cell plate for endocrine disrupting chemicals (EDCs) detection, like estrogenic activity, in waterish solution. This platform technology consists of four independent micro flow units made of polydimethylsiloxane (PDMS) and glass, which enables a selective detection of up to four species of the EDCs per one-way chip containing the corresponding immobilized receptor. The concept of the detection is based on direct fluorescence analysis. In order to found out the electrical parameters of the microfluidic system electroluminescence (EL) measurements as a function of the concentration of the QD800 dye were investigated. Finally, the microfluidic device was attached to the flow control system. Different edge filters were tested in order to attenuate the MOSLED light signal and to maximize the QD800 dye signal at 800nm which works best for a 780nm edge filter. Measurements using an integrated photo diode as detector were performed to point out the relationship between the dark and photo current.
ISSN:1877-7058
1877-7058
DOI:10.1016/j.proeng.2011.12.292