1,25-dihydroxyvitamin D3 inhibits the clonogenic growth of transformed cells via its receptor

• Published in: Biochemical and biophysical research communications Vol. 139; no. 1; pp. 136 - 143
• Main Authors: Haussler, C.A, Marion, S.L, Pike, J.W, Haussler, M.R
• Format: Journal Article
• Language: English
• Published: San Diego, CA Elsevier 29.08.1986
• Subjects: 1,25-DIHYDROXYCHOLECALCIFEROL; Antineoplastic Agents - pharmacology; Applied sciences; Calcitriol - pharmacology; Cell Line; CELLS; CELLULE; CELULAS; DNA - metabolism; Exact sciences and technology; GROWTH RATE; INDICE DE CRECIMIENTO; Neoplastic Stem Cells - drug effects; Other techniques and industries; Receptors, Calcitriol; Receptors, Steroid - analysis; Receptors, Steroid - physiology; TAUX DE CROISSANCE; VITAMIN D; Vitamin D - pharmacology; VITAMINA D; VITAMINE D
• ISSN: 0006-291X; 1090-2104
• DOI: 10.1016/S0006-291X(86)80090-0

Anchorage-independent growth in soft agar is a unique property of transformed cells which is known to be correlated with tumorigenicity. We report here that 1,25-dihydroxyvitamin D3 suppresses colony formation by a number of cultured cancer cell lines in soft agar in a dose dependent manner with an ID50 of 5-7 X 10(-10) M. This effect is also achieved with analogues of 1,25-dihydroxyvitamin D3 in accordance with their binding affinity for the hormone's receptor. Only cells with 1,25-dihydroxyvitamin D3 receptor protein are inhibited in their colony formation by vitamin D analogs indicating that the hormone receptor complex may be integrally involved in the in vitro suppression of the anchorage-independent phenotype.