Temperature has a strong impact on the abundance and community structure of comammox Nitrospira in an Ultisol

• Published in: Journal of soils and sediments Vol. 22; no. 10; pp. 2593 - 2603
• Main Authors: Feng, Mengmeng, He, Zi-Yang, Fan, Jianbo, Ge, An-Hui, Jin, Shengsheng, Lin, Yongxin, He, Ji-Zheng
• Format: Journal Article
• Language: English
• Published: Berlin/Heidelberg Springer Berlin Heidelberg 01.10.2022; Springer Nature B.V
• Subjects: Abundance; Agricultural ecosystems; agroecosystems; Ammonia; Community structure; Earth and Environmental Science; Ecophysiology; Environment; Environmental Physics; Experiments; Influence; Laboratories; Low temperature; Microorganisms; Nitrification; Nitrogen; Nitrospira; phylotype; Sec 1 • Soil Organic Matter Dynamics and Nutrient Cycling • Research Article; Sediments; Soil Science & Conservation; Soil sciences; Soil temperature; Soil testing; Soils; Temperature; Temperature effects; Terrestrial ecosystems; Ultisols; Temperature change; Nitrogen addition; Comammox; Acidic soil
• ISSN: 1439-0108; 1614-7480
• DOI: 10.1007/s11368-022-03261-5

Purpose Temperature is a key factor influencing the growth and activities of microorganisms and their associated biogeochemical processes including nitrification. However, limited studies have been performed to evaluate the response of the newly discovered comammox Nitrospira to temperature change and nitrogen (N) addition in agroecosystems, which has hindered our understanding of the ecophysiology and environmental adaption of comammox Nitrospira in agroecosystems. Materials and methods We set up a microcosm experiment with 3 temperature treatments (15 °C, 25 °C, and 35 °C) × 3 levels of N addition (0 mg kg −1 , 25 mg kg −1 , and 50 mg kg −1 ) in an acidic Ultisol. We explored the impact of temperature and N addition on the abundance and community structure of comammox Nitrospira . Results and discussion The addition of N and increasing temperature generally increased the net nitrification rate. The comammox Nitrospira clade A abundance decreased with the increase of temperature, indicating that clade A might favor environments with relatively lower temperature. However, the addition of N did not significantly influence the abundance of comammox Nitrospira clade A. Moreover, temperature had a stronger effect than N addition on the community structure of comammox Nitrospira . Clade A.2.1 were the exclusively dominant comammox Nitrospira phylotype in the tested soils, and were not affected by temperature and N addition, suggesting that temperature influenced comammox Nitrospira mainly through regulating the internal subclades of clade A.2.1. Conclusions Our findings provide novel evidence that temperature had a stronger effect than N addition on the abundance and community structure of comammox Nitrospira and suggest that comammox Nitrospira are temperature sensitive and favor relatively lower temperature in the acidic Ultisols.