The adjunctive effect of polydeoxyribonucleotide on bone formation in alveolar ridge preservation: A pre‐clinical in vivo study

• Published in: Journal of clinical periodontology Vol. 51; no. 8; pp. 1034 - 1043
• Main Authors: Ko, Young‐Chang, Lee, Jungwon, Urban, Istvan, Seol, Yang‐Jo, Lee, Yong‐Moo, Koo, Ki‐Tae
• Format: Journal Article
• Language: English
• Published: Oxford, UK Blackwell Publishing Ltd 01.08.2024
• Subjects: Allografts; Alveolar bone; Alveolar Process - diagnostic imaging; Alveolar Process - drug effects; alveolar ridge preservation; Animals; Blood vessels; Bone growth; Bone healing; bone regeneration; Bone Regeneration - drug effects; Bone Substitutes - pharmacology; Bone Substitutes - therapeutic use; Collagen; Collagen - pharmacology; Dogs; Imaging, Three-Dimensional - methods; Male; Mandible - diagnostic imaging; Mandible - drug effects; Mandible - surgery; Membranes, Artificial; Osteogenesis; Osteogenesis - drug effects; polydeoxyribonucleotide; Polydeoxyribonucleotides - pharmacology; Polydeoxyribonucleotides - therapeutic use; Premolars; Random Allocation; Regeneration; Statistical analysis; Tomography; Tooth Extraction; Tooth Socket - diagnostic imaging; Tooth Socket - drug effects; Tooth Socket - surgery; Wound Healing - drug effects; X-Ray Microtomography; bone regeneration; alveolar ridge preservation; polydeoxyribonucleotide
• ISSN: 0303-6979; 1600-051X; 1600-051X
• DOI: 10.1111/jcpe.13988

Aim This study investigated the adjunctive effect of polydeoxyribonucleotide (PDRN) on bone formation in alveolar ridge preservation (ARP) sockets. Materials and Methods Both mandibular second, third and fourth premolars of eight beagle dogs were randomly divided into ARP and ARP/PDRN groups. Following tooth extraction, ARP procedures were conducted using collagenized alloplastic graft material and bilayer collagen membrane soaked with normal saline (ARP group) or PDRN (ARP/PDRN group) for 10 min before application. Both groups were also randomly allocated to 2‐, 4‐ or 12‐week healing subgroups. The primary endpoint of this study was to compare histomorphometric differences between ARP and ARP/PDRN. The secondary endpoints of this study were to compare micro‐CT analysis and three‐dimensional volumetric measurement between the two groups. Results In the histomorphometric analysis, the ARP/PDRN group exhibited greater new bone formation at coronal, middle and total position compared with the ARP group at 2‐week healing. The number of newly formed blood vessels was higher in the ARP/PDRN group than in the ARP group at 2‐ and 4‐week healing. In micro‐CT analysis, the mean new bone volume/total bone volume between ARP and ARP/PDRN was statistically significant at 2‐week healing. Ridge volume alterations were significantly decreased in the ARP/PDRN group during entire healing time compared with the ARP group, especially on the buccal side. Conclusions The application of PDRN in ARP might provide additional benefits for early bone regeneration and maintenance of buccal ridge volume.