NO Production in Cultured Equine Cells Stimulated by LPS and Cytokines

Culture cells from a horse were stimulated by LPS and cytokines, and nitric oxide (NO) production was rendered detectable by a fluorescent measurement reagent. Increase of NO production was observed over time in the LPS-and cytokine-stimulated cells after the addition of l-arginine, a substrate for...

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Bibliographic Details
Published inJournal of Equine Science Vol. 11; no. 1; pp. 15 - 18
Main Authors ISHIDA, Nobushige, SATO, Fumio, HIROTA, Kei-ichi, HASEGAWA, Telhisa
Format Journal Article
LanguageEnglish
Published Utsunomiya-shi Japanese Society of Equine Science 2000
Japan Science and Technology Agency
Subjects
culture-cell
i-NOS
LPS
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Summary:Culture cells from a horse were stimulated by LPS and cytokines, and nitric oxide (NO) production was rendered detectable by a fluorescent measurement reagent. Increase of NO production was observed over time in the LPS-and cytokine-stimulated cells after the addition of l-arginine, a substrate for NO. NO production was observed to increase slightly over time, even in cells not stimulated by the addition of l-arginine. The addition of l-NMMA (an i-NOS inhibitor) along with l-arginine to the LPS-and cytokine-stimulated cells was found to markedly suppress NO synthesis. Enhancement of fluorescence intensity depended on LPS levels, suggesting that NO production may also depend on LPS levels.
ISSN:1340-3516
1347-7501
DOI:10.1294/jes.11.15