Fluid-phase transcytosis in the primate epididymis in vitro and in vivo

Ligated tubules from the corpus epididymidis of men and monkeys were incubated in medium containing horseradish peroxidase (HRP) as a marker for fluid-phase endocytosis. HRP was localized by light and electron microscopy after 0, 15, 30 and 60 min of incubation. Movement between the cells was preven...

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Bibliographic Details
Published inInternational journal of andrology Vol. 12; no. 5; p. 384
Main Authors Yeung, C H, Cooper, T G, Weinbauer, G F, Bergmann, M, Kleinhans, G, Schulze, H, Nieschlag, E
Format Journal Article
LanguageEnglish
Published England 01.10.1989
Subjects
Aged
Animals
Biological Transport - physiology
Endocytosis - physiology
Epididymis - cytology
Epididymis - metabolism
Horseradish Peroxidase - analysis
Horseradish Peroxidase - metabolism
Humans
In Vitro Techniques
Macaca fascicularis
Male
Time Factors
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Summary:Ligated tubules from the corpus epididymidis of men and monkeys were incubated in medium containing horseradish peroxidase (HRP) as a marker for fluid-phase endocytosis. HRP was localized by light and electron microscopy after 0, 15, 30 and 60 min of incubation. Movement between the cells was prevented by tight junctions, but bypass of this barrier was apparently achieved by an intracellular vesicular mechanism leading to a time-dependent appearance of HRP in the lumen. Uptake of HRP into basal cells and capture by the lysosomal apparatus of principal cells were also observed. HRP-filled vesicles also appeared in the basal, mid and apical cytoplasm of epithelial cells in the caput 1 h after injection of the tracer into the epididymal circulation of the monkey, suggesting that this pathway also operates in vivo.
ISSN:0105-6263
DOI:10.1111/j.1365-2605.1989.tb01327.x