Regulation of the primary in vitro response to TNP-polymerized ovalbumin by T suppressor cells induced by ovalbumin feeding

Spleen cells from DBA/2 mice that received a single feeding of 20 mg of ovalbumin (OVA) 7 days previously were specifically hyporesponsive to primary in vitro challenge with the thymic-dependent antigen TNP-polymerized ovalbumin (TNP-POL-OVA). The tolerance observed in spleen cells from OVA-fed anim...

Full description

Saved in:
Bibliographic Details
Published inThe Journal of immunology (1950) Vol. 132; no. 6; pp. 2783 - 2789
Main Authors Cowdery, JS, Johlin, BJ
Format Journal Article
LanguageEnglish
Published Bethesda, MD Am Assoc Immnol 01.06.1984
American Association of Immunologists
Subjects
Administration, Oral
Analysis of the immune response. Humoral and cellular immunity
Animals
Antibody Formation
Antigens - administration & dosage
Antigens - immunology
Biological and medical sciences
Cell interactions
Female
Fundamental and applied biological sciences. Psychology
Fundamental immunology
Immune Tolerance
Immunobiology
Lymphocyte Activation
Macromolecular Substances
Mice
Mice, Inbred DBA
Ovalbumin - administration & dosage
Ovalbumin - immunology
Spleen - immunology
T-Lymphocytes, Regulatory - immunology
Ovalbumin
Rodentia
Polymer
Immune regulation
In vitro
Immunological method
Primary response
Vertebrata
Mammalia
Mouse
Animal
T-Lymphocyte
Suppressive cell
Online AccessGet full text

Cover

More Information
Summary:Spleen cells from DBA/2 mice that received a single feeding of 20 mg of ovalbumin (OVA) 7 days previously were specifically hyporesponsive to primary in vitro challenge with the thymic-dependent antigen TNP-polymerized ovalbumin (TNP-POL-OVA). The tolerance observed in spleen cells from OVA-fed animals was dependent upon OVA-specific T suppressor cells, because splenic T cells from OVA-fed mice suppressed the primary response to TNP-POL-OVA of cultures containing normal T and B cells. The tolerance and suppression was OVA specific, because spleen cells from OVA-fed animals responded well to other antigens (including TNP on another carrier), and splenic T cells from OVA-fed mice did not affect the response of normal T and B cells to sheep erythrocytes. These data confirm the existence of T suppressor cells after OVA feeding and provide a direct means of assaying their activity in a primary in vitro response.
Bibliography:ObjectType-Article-2
SourceType-Scholarly Journals-1
ObjectType-Feature-1
content type line 23
ObjectType-Article-1
ObjectType-Feature-2
ISSN:0022-1767
1550-6606
DOI:10.4049/jimmunol.132.6.2783