Reverse Transcriptase Sequencing of 16S Ribosomal RNA from Faenia rectivirgula, Pseudonocardia thermophila and Saccharopolyspora hirsuta, Three Wall Type IV Actinomycetes Which Lack Mycolic Acids

• Published in: Journal of general microbiology Vol. 134; no. 4; pp. 961 - 966
• Main Authors: Embley, T. Martin, Smida, Jan, Stackebrandt, Erko
• Format: Journal Article
• Language: English
• Published: London Soc General Microbiol 01.04.1988; New York, NY Cambridge University Press
• Subjects: Actinomycetales - classification; Actinomycetales - genetics; Bacteriology; Base Sequence; Biological and medical sciences; Fundamental and applied biological sciences. Psychology; Microbiology; Molecular Sequence Data; Mycolic Acids - genetics; Phylogeny; RNA, Bacterial - classification; RNA, Ribosomal - classification; RNA, Ribosomal, 16S - classification; RNA-Directed DNA Polymerase; Systematics; Gene; Nucleotide sequence; Enzyme; Taxonomy; DNA polymerase RNA-dependent; Chemotaxonomy; 16S-RNA; Bacteria; Homology; Actinomycetes; Phylogeny; Comparative study
• ISSN: 0022-1287; 1350-0872; 1465-2080
• DOI: 10.1099/00221287-134-4-961

Department of Paramedical Sciences, North East London Polytechnic, London E15 4LZ, UK Institut für Allgemeine Mikrobiologie, Christian-Albrechts-Universität, 2300 Kiel, FRG ABSTRACT SUMMARY: The phylogenetic relationships of three mycolateless wall type IV actinomycetes, Faenia rectivirgula, Pseudonocardia thermophila and Saccharopolyspora hirsuta , were examined using reverse transcriptase sequencing of 16S ribosomal RNA. The sequences generated were aligned and the level of sequence homology calculated. The homology values were then used to produce a phylogenetic tree and to estimate S AB values for the construction of a dendrogram. Both analyses show the three taxa to be closely related genera which form a distinct subdivision within the broader phylogenetic grouping defined by Mycobacterium, Dactylosporangium and their relatives.