Ultra-trace determination of S-nitrosothiols in blood samples by spectrofluorimetry with 8-(3′,4′-diaminophenyl)-difluoroboradiaza- s-indacene

• Published in: Spectrochimica acta. Part A, Molecular and biomolecular spectroscopy Vol. 69; no. 2; pp. 437 - 442
• Main Authors: Huang, Ke-Jing, Xu, Chun-Xuan, Xie, Wan-Zhen, Zhang, Hua-Shan, Wang, Hong
• Format: Journal Article
• Language: English
• Published: England Elsevier B.V 01.02.2008
• Subjects: 8-(3′,4′-Diaminophenyl)-difluoroboradiaza- s-indacence; Blood samples; Boron Compounds - chemistry; Glutathione - analogs & derivatives; Glutathione - chemistry; Heterocyclic Compounds, 3-Ring - chemistry; Humans; Ions - chemistry; Molecular Structure; Nitro Compounds - chemistry; S-Nitrosothiols; S-Nitrosothiols - blood; S-Nitrosothiols - chemistry; Spectrofluorimetry; Spectrometry, Fluorescence - methods; Blood samples; 8-(3′,4′-Diaminophenyl)-difluoroboradiaza- s-indacence; S-Nitrosothiols; Spectrofluorimetry
• ISSN: 1386-1425
• DOI: 10.1016/j.saa.2007.04.020

Increasing evidence suggests that S-nitrosothiols (RSNO) may represent naturally occurring nitric oxide (NO) surrogates and function as intermediates in NO metabolism. In this work, a simple, sensitive, and selective micromethod is developed and validated for quantification of RSNO. A fluorescent probe 8-(3′,4′-diaminophenyl)-difluoroboradiaza- s-indacence (DABODIPY) is firstly used to label RSNO. The derivatization reaction is performed in aqueous medium at 30 °C for 15 min in the presence of 6 × 10 −5 mol L −1 Hg 2+ and the derivative is detected by fluorescence at λ ex/ λ em = 500/510 nm. A linear function of concentration in the range of (2.0–600.0) × 10 −8 mol L −1 is observed with a correlation coefficient of 0.9992 and detection limit of 1.2 × 10 −9 mol L −1 (S/N = 3). This technique has been successfully applied to quantify RSNO in some human blood samples including healthy persons and patients suffering from cardiovascular diseases.