IL10 and CXCL10 mRNA expression in food protein–induced enterocolitis syndrome

• Published in: Cytokine (Philadelphia, Pa.) Vol. 182; p. 156720
• Main Authors: Galliano, Ilaria, Montanari, Paola, Monti, Giovanna, Dini, Maddalena, Calvi, Cristina, Clemente, Anna, Pau, Anna, Gambarino, Stefano, Bergallo, Massimiliano
• Format: Journal Article
• Language: English
• Published: England Elsevier Ltd 01.10.2024
• Subjects: Case-Control Studies; Chemokine CXCL10 - blood; Chemokine CXCL10 - genetics; Child; Child, Preschool; Children; CXCL10; Enterocolitis - genetics; Enterocolitis - immunology; Female; Food Hypersensitivity; Food protein–induced enterocolitis syndrome (FPIES); Humans; IL10; Infant; Interleukin-10 - blood; Interleukin-10 - genetics; Male; RNA, Messenger - genetics; RNA, Messenger - metabolism; Syndrome; IL10; CXCL10; Children; Food protein–induced enterocolitis syndrome (FPIES)
• ISSN: 1043-4666; 1096-0023; 1096-0023
• DOI: 10.1016/j.cyto.2024.156720

Food protein-induced enterocolitis syndrome (FPIES) is a rare non-IgE-mediated food allergy that mainly impacts babies and 7toddlers. The exact mechanism of FPIES is not completely understood. By studying the expression of IL-10 and CXCL10 in pediatric FPIES patients, researchers can gain insights into the immune mechanisms underlying this disorder. Peripheral venous blood was collected and subsequently stabilized with RNA pro. Total RNA was extracted and mRNA levels of CXCL10 and IL-10 was determined with real time PCR. Children with FPIES had significantly higher values than the healthy control group (HC) for CXCL10 while FPIES had a significant lower values than the control group for IL-10. Our results show a high production of CXCL10 and a concomitant reduced production of IL-10 in FPIES subjects who have not yet reached tolerance. These data may represent a molecular diagnostic marker for FPIES.