Growth of human breast cancer cell lines is inhibited by the somatostatin analog BIM23014

Somatostatin has been shown to lower plasma levels of various hormones and growth factors involved in regulation of the growth of human breast cells. In the present study we examined the ability of the somatostatin octapeptide analog BIM23014 to modulate the in vitro growth of five human breast cell...

Full description

Saved in:
Bibliographic Details
Published inEndocrinology (Philadelphia) Vol. 129; no. 1; p. 323
Main Authors Prévost, G, Foehrlé, E, Thomas, F, Pihan, I, Veber, N, Starzec, A, Israël, L
Format Journal Article
LanguageEnglish
Published United States 01.07.1991
Subjects
Binding, Competitive
Blood
Breast Neoplasms - pathology
Cell Division - drug effects
Cross-Linking Reagents
Culture Media
DNA - biosynthesis
Estradiol - pharmacology
Humans
Insulin - pharmacology
Kinetics
Peptides, Cyclic - pharmacology
Progesterone - pharmacology
Somatostatin - analogs & derivatives
Tumor Cells, Cultured
Online AccessGet more information

Cover

More Information
Summary:Somatostatin has been shown to lower plasma levels of various hormones and growth factors involved in regulation of the growth of human breast cells. In the present study we examined the ability of the somatostatin octapeptide analog BIM23014 to modulate the in vitro growth of five human breast cell lines: HBL100, Hs578T, MDAMB231, T47D, and MCF7. BIM23014 inhibited the growth of the two steroid-dependent cell lines, MCF7 and T47D, in a dose-related manner. This inhibitory effect was only observed when MCF7 and T47D cells were cultivated in medium containing steroid-depleted serum. The growth of a MCF7 variant capable of growth in serum-free medium was also inhibited by BIM23014, indicating that serum factors are not required for this inhibition. In the serum-free medium, the addition of estradiol before or during treatment with BIM23014 abolished its inhibitory effects on cell growth. The studies including time course, competitive inhibition, and cross-linking of iodinated BIM23014 to its receptor revealed a specific binding on MCF7 cells and showed a single 57,000 mol wt protein band in sodium dodecyl sulfate-polyacrylamide gel electrophoresis. These results support the hypothesis that BIM23014 inhibits the growth of steroid-receptor positive cells of human breast cancers through its own receptor in estradiol-free conditions.
ISSN:0013-7227
1945-7170
DOI:10.1210/endo-129-1-323