ADF10 shapes the overall organization of apical actin filaments by promoting their turnover and ordering in pollen tubes

• Published in: Journal of cell science Vol. 130; no. 23; pp. 3988 - 4001
• Main Authors: Jiang, Yuxiang, Wang, Juan, Xie, Yurong, Chen, Naizhi, Huang, Shanjin
• Format: Journal Article
• Language: English
• Published: England The Company of Biologists Ltd 01.12.2017
• Subjects: Actin; Actin Cytoskeleton - metabolism; Actin Depolymerizing Factors - genetics; Actins - metabolism; Arabidopsis - metabolism; Arabidopsis Proteins - genetics; Depolymerization; Filaments; Gene Expression Regulation, Plant; Mutants; Pollen; Pollen - genetics; Pollen - metabolism; Pollen Tube - metabolism; Pollen tubes; Protein Transport - genetics; Tubes; Vesicles; Apical actin structure; ADF; Pollen tube; Actin dynamics; TALEN; Actin filament severing
• ISSN: 0021-9533; 1477-9137
• DOI: 10.1242/jcs.207738

Here, we show that ADF10 plays an important role in shaping the overall organization of apical actin filaments by promoting their turnover and ordering. ADF10 severs and depolymerizes actin filaments and is distributed throughout the entire pollen tube. In mutants, severing and monomer dissociation events for apical actin filaments are reduced, and the apical actin structure extends further toward the tube base than in wild-type tubes. In particular, the percentage of apical actin filaments that form large angles to the tube growth axis is much higher in pollen tubes, and the actin filaments are more randomly distributed, implying that ADF10 promotes their ordering. Consistent with the role of apical actin filaments in physically restricting the movement of vesicles, the region in which apical vesicles accumulate is enlarged at the tip of pollen tubes. Both tipward and backward movements of small vesicles are altered within the growth domain of pollen tubes. Thus, our study suggests that ADF10 shapes the organization of apical actin filaments to regulate vesicle trafficking and pollen tube growth.