Characterization of Xenopus laevis cDNA clones of the c-ets-1 proto-oncogene

• Published in: Nucleic acids research Vol. 18; no. 17; p. 5298
• Main Authors: STIEGLER, P, WOLFF, C.-M, BALTZINGER, M, HIRTZLIN, J, SENAN, F, MEYER, D, GHYSDAEL, J, STEHELIN, D, BEFORT, N, REMY, P
• Format: Journal Article
• Language: English
• Published: Oxford Oxford University Press 11.09.1990
• Subjects: Amino Acid Sequence; Animals; Base Sequence; Biological and medical sciences; c-ets-1; Cloning, Molecular; DNA - genetics; Fundamental and applied biological sciences. Psychology; Genes. Genome; Molecular and cellular biology; Molecular genetics; Molecular Sequence Data; Oocytes - metabolism; Proto-Oncogene Proteins - genetics; Proto-Oncogene Proteins c-ets; Proto-Oncogenes; Sequence Homology, Nucleic Acid; Transcription Factors; Xenopus laevis; Xenopus laevis - genetics; Vertebrata; Complementary DNA; Nucleotide sequence; C-Onc gene; Xenopus laevis; Amphibia; Salientia; Molecular cloning
• ISSN: 0305-1048; 1362-4962
• DOI: 10.1093/nar/18.17.5298

Two different cDNAs of the Xenopus c-ets-1 proto-oncogene were obtained by screening a cDNA library constructed from stage I, II oocyte mRNAs with a heterologous probe derived from the chicken c-ets-2 gene. One of these cDNAs (Xl-ets-1a) of 4.1 kb encodes a 438 amino acids polypeptide. The other cDNA, Xl-ets-1b (1.3 kb) is restricted to part of the coding sequence and a shorter 3'-non coding region. The Xl-ets-1b cDNA differs from the corresponding region in Xl-ets-1a in 7.5% of the nucleotides. Such a nucleotide divergence as well as the existence of short deletions in the 3'-non coding region of Xl-ets-1b suggest that Xl-ets-1a and Xl-ets-1b arise from two different ets-1 genes in Xenopus laevis .