Localization of the gene encoding steroid hydroxylase cytochrome P-450 from Rhizopus nigricans inside a HindIII fragment of genomic DNA

• Published in: Biochemical and biophysical research communications Vol. 178; no. 3; pp. 1078 - 1083
• Main Authors: Breskvar, Katja, Črešnar, Bronislava, Plaper, Andreja, Hudnik-Plevnik, Tamara
• Format: Journal Article
• Language: English
• Published: San Diego, CA Elsevier Inc 15.08.1991; Elsevier
• Subjects: Amino Acid Sequence; Analytical, structural and metabolic biochemistry; Base Sequence; Biological and medical sciences; Cytochrome P-450 Enzyme System - genetics; Deoxyribonuclease HindIII; DNA, Fungal - genetics; Enzymes and enzyme inhibitors; ESTEROIDES; Fundamental and applied biological sciences. Psychology; GENE; Gene Library; GENES; Genes, Fungal; METALLOPROTEINE; METALPROTEINAS; Molecular Sequence Data; NUCLEOTIDE; NUCLEOTIDOS; Oxidoreductases; OXIDORREDUCTASAS; OXYDOREDUCTASE; Restriction Mapping; RHIZOPUS; Rhizopus - enzymology; Rhizopus - genetics; Steroid Hydroxylases - genetics; STEROIDE; Fungi; Gene; Nucleotide sequence; Cytochrome P450; Rhizopus; Phycomycetes; Restriction map; Aminoacid sequence; Thallophyta
• ISSN: 0006-291X; 1090-2104
• DOI: 10.1016/0006-291X(91)91002-T

The gene encoding steroid inducible cytochrome P450 of Rhizopus nigricans ATCC 6227b has been found inside a HindIII fragment of the genomic DNA by hybridization with a partial length cDNA probe. The latter was isolated by immunoscreening a cDNA library prepared in the λgtll expression system and identified on the basis of inducibility and sequence analysis. The nucleotide sequence of the cDNA probe revealed a coding sequence for the heme binding segment characteristic of the P450 gene family.