Differential expression of two interferon- γ genes in common carp ( Cyprinus carpio L.)

• Published in: Developmental and comparative immunology Vol. 32; no. 12; pp. 1467 - 1481
• Main Authors: Stolte, Ellen H., Savelkoul, Huub F.J., Wiegertjes, Geert, Flik, Gert, Lidy Verburg-van Kemenade, B.M.
• Format: Journal Article
• Language: English
• Published: United States Elsevier Ltd 2008
• Subjects: Amino Acid Sequence; Animals; B-lymphocyte function; Base Sequence; Carps - genetics; Carps - immunology; Cattle; Cells, Cultured; Common carp; Cytokine; Dogs; GATA3; Gene Expression Regulation - physiology; Humans; IFN- γ; Interferon-gamma - biosynthesis; Interferon-gamma - genetics; Interferon-gamma - isolation & purification; Mice; Molecular Sequence Data; Protein Isoforms - biosynthesis; Protein Isoforms - genetics; Protein Isoforms - isolation & purification; Rabbits; Rats; Sequence Alignment; T-bet; T-lymphocyte function; Teleost; Teleost; Common carp; T-lymphocyte function; Cytokine; GATA3; B-lymphocyte function; T-bet; IFN- γ
• ISSN: 0145-305X; 1879-0089
• DOI: 10.1016/j.dci.2008.06.012

Two interferon gamma (IFN- γ) genes are expressed in immune cells of teleost fish and are potentially implicated in B- and T-lymphocyte responses. IFN- γ-2 shows structural and functional characteristics to other vertebrate IFN- γ genes and is associated with T-lymphocyte function. Expression profiling shows IFN- γ-2 upregulation in T-lymphocytes after phytohemagglutinin (PHA) stimulation in vitro. Unexpectedly, we found IFN- γ-1, which is structurally different from IFN- γ-2, to be expressed in lipopolysacharide (LPS)-stimulated IgM + (B- lymphocyte enriched) fractions. Expression of T-box transcription factor T-bet, but not of GATA-binding protein 3 (GATA3), correlated with expression of both IFN- γ genes. In-vivo parasite infection, but as predicted not zymosan-induced inflammation, resulted in concomitant upregulation of T-bet and IFN- γ-2. This corroborates a genuine T-lymphocyte associated role for IFN- γ-2.